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绵羊肺腺瘤病毒囊膜基因表面蛋白的表达及其单克隆抗体的制备 被引量：3

Development of monoclonal antibodies against the env gene of Jaagsiekte sheep retrovirus
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摘要 在E.coli BL21中诱导表达含有绵羊肺瘤病毒囊膜表面蛋白(SU)重组质粒pGEX-4T-1-SU,表达的GST融合蛋白经纯化后用佐剂乳化,作为抗原免疫BALB/c小鼠,并进行细胞融合、克隆化、ELISA法初步筛选抗SU单抗。最终获得了3株抗SU的单抗,为深入探讨JSRV的分子生物学特性及研制诊断试剂盒打下了基础。 Monoclonal antibodies against Jaagsiekte sheep retrovirus were prepared using recombinant env protein expressed from plasmid pGEX-4T-1-SU in E.coli BL21. BALB/c mice were immunized and three McAb clones against SU domain of the env protein were developed by standard hybridoma technique and identified by ELISA. These McAbs provide potential useful tools for diagnosis of JSRV and for the further study of JSRV at molecular level.
作者 刘霄卉 斯日古楞 罗军荣 周建华 马学恩
机构地区 内蒙古农业大学动物科学与医学学院 中国农业科学院哈尔滨兽医研究所
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2008年第6期469-472,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 国家自然科学基金项目(30260083) 教育部高等学校博士学科点专项科研基金项目(20040129001)
关键词 绵羊肺腺瘤病毒 囊膜基因 表面蛋白 原核表达 单克隆抗体 JSRV env gene SU eukaryocyte expression McAb
分类号 S852.659 [农业科学—基础兽医学]
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参考文献13

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二级参考文献37

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共引文献20

同被引文献27

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二级引证文献16

中国预防兽医学报
2008年 第6期

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