幽门螺旋杆菌感染后血清学改变的研究

The Serological Changes after Helicobacter Pylori Infection

目的分析幽门螺旋杆菌感染后血清中所产生的H.pylori-IgG亚型、CagA-IgG亚型与临床转归的关系。方法以ELISA方法测定126位患者(42位消化性溃疡患者,84位慢性胃炎患者)血清H.pylori-IgG亚型和CagAIgG亚型。结果(1)H.pylori-IgG以IgG1和IgG2为主,IgG3和IgG4水平很低;CagA-IgG以IgG1和IgG3为主,IgG2水平很低,IgG4未测出;(2)消化性溃疡患者H.pylori-IgG3水平高于慢性胃炎患者,其他抗体水平无显著差异;(3)CagA-IgG阳性组患者的HP-IgG抗体及其亚型IgG1、IgG3均明显高于CagA-IgG阴性组,P<0.01。结论消化性溃疡患者H.pylori-IgG3水平高于慢性胃炎患者,由于其固定补体的作用,可能在溃疡的发病中具有一定作用。细菌是否表达CagA对宿主的免疫反应具有一定的影响,同临床转归可能相关。

Objective： This study is aiming to show the profile of IgG subclasses after H. pylori infection and its association with clinical outcomes. Methods： 126 patiets （42 cases were peptic ulcer, 82 cases were chronic gastritis） were included. H. pylori-IgG subtypes and CagA-IgG subtypes in sera were tested by ELISA. Results： 1, IgG1 and IgG2 were dominant of H. pylori-IgG subtypes, The levels ofH. pylori-IgG3 and IgG4 were very low, IgG1 and IgG3 were predominating in CagA-IgG, CagA-IgG4 was undetectable, 2, Patients with peptic ulcer had higher level of H,pylori-lgG3 than patients with chronic gastritis. 3, Patients with CagA-IgG positive have higher level of H.pylori- IgG and its subtype H.pylori -IgG1 IgG3 Conclusion： The patients with peptic nicer had higher level of H. pylori IgG3, which maybe contribute to pathogenesis as IgG3 has the strong ability to fix complements. Whether helicohacter pylori is CagA positive or not has effect on host immune respone and is related to clinical outcomes after infection.