摘要
本研究应用犬细小病毒抗原快速检测试剂盒、血凝/血凝抑制试验和PCR方法对120份具有腹泻/呕吐症状的患犬粪便样本进行了犬细小病毒检测。结果显示,3种方法检测阳性份数分别为60,49,68。犬细小病毒抗原快速检测试剂盒与血凝/血凝抑制(HA/HI)试验相比较,敏感性、特异性及总符合率分别为100.0%,84.5%,90.8%;与PCR法相比较,敏感性、特异性及总符合率分别为85.3%,96.2%,90.0%。从试验结果看,HA/HI法具有较高的特异性,但敏感性较低;PCR具有高度敏感性和特异性,但不适合临床推广;细小病毒抗原快速检测试剂盒,在正确操作下结果可靠,是临床诊断犬细小病毒感染的首选方法。
Three diagnostic methods for canine parvovirus （CPV） infection were employed to test 120 feces samples collected from dogs with diarrhea and/or vomit in this study. The CPV positive samples were 60, 49 and 68, respectively by detecting CPV antigen or nucleic acid using Antigen Rapid CPV Ag Test Kit, haemagglutination/haemagglutination inhibition （HA/HI） and PCR assay. The results of these three methods were evaluated. The percentages showed 100. 0% , 84. 5% and 90. 8% , respectively in relative sensitivity, specificity and overall agreement between Antigen Rapid CPV Ag Test Kit and HA/HI, and 85.3% , 96. 2% and 90. 0 % , respectively between Antigen Rapid CPV Ag Test Kit and PCR. These results indicated that HA/HI was more specific than Antigen Rapid CPV Ag Test Kit but had a relatively lower sensitivity. PCR was sensitive and specific, but it was not a suitable test in clinic. The Antigen Rapid CPV Ag Test Kit was the first choice for detecting CPV infection if correctly used.
出处
《畜牧与兽医》
北大核心
2008年第6期31-35,共5页
Animal Husbandry & Veterinary Medicine
