兔耳风属3个近缘种ISSR分子鉴别

ISSR molecular identification for three species in Ainsliaea

采用ISSR分子标记技术,对菊科兔耳风属杏香兔耳风(Ainsliaea fragrans Champ)、红背兔耳风(A.rubrifolia Franc)和铁灯兔耳风(A.macroclinidioides Hag)等3个极易混淆的近缘种进行了分子鉴别。从100条ISSR UBC系列引物中筛选出20条多态性好的引物进行检测,其中利用引物UBC807、UBC826、UBC834、UBC843、UBC853、UBC895的扩增特异性条带制成ISSR指纹图谱,完全可以用于3个近缘种的鉴别。此外,基于相似系数的UPGMA聚类分析显示,杏香兔耳风与红背兔耳风之间亲缘关系近,而与铁灯兔耳风的亲缘关系则相对较远。

Three difficultly-divisional species, i. e. , Ainsliaea fragrans, A. macroclinidioides and A. rubrifolia were studied using the ISSR molecular identification system. Twenty primers were screened out from 100 ISSR UBC. They could generate reproducible polymorphic fragments. The particular bands were expanded to make the ISSR fingerprint by primer UBC807, UBC826, UBC834, UBC843, UBC853, and UBC895. It could be used to discriminate three species. Furthermore, according to the alike coefficient UPGMA showed that the relative relation of A. fragrans and A. rubrifolia was closer than that of A. fragrans and A. maeroelinidioide. The research could be responded for the conservancy and development of the chinese herbal medicine resource, and providing a technical support of building up the stable quality evaluation system of chinese herbal medicine.