摘要
目的研究可溶性血管内皮生长因子受体1(sFlt-1)和血管内皮生长因子(VEGF)在子痫前期患者血清中的浓度变化,以及子宫胎盘床螺旋动脉的相应变化,探讨sFlt-1和VEGF在子痫前期中的作用。方法采用酶联免疫吸附试验(ELISA)分别测定轻度子痫前期、重度子痫前期患者及正常妊娠妇女外周血清中sFlt-1和VEGF的浓度。同时按照Robertson方法,获取胎盘床活检标本,在光学显微镜下观察子痫前期组与对照组胎盘床螺旋动脉的生理性变化。结果(1)子痫前期组各组外周血清中sFlt-1水平明显高于对照组(P<0.01),子痫前期重度组高于子痫前期轻度组(P<0.05);(2)子痫前期组各组外周血血清中VEGF水平明显低于对照组(P<0.05),子痫前期重度组低于子痫前期轻度组(P<0.05)。正常妊娠组与子痫前期组胎盘床蜕膜螺旋动脉的生理性改变差异无统计学意义(P>0.05)。子痫前期组肌层螺旋动脉的生理性改变明显少于正常妊娠组,2组差别有统计学意义(P<0.05)。结论外周血中升高的sFlt-1和降低的VEGF以及相应的肌层螺旋动脉生理性改变可能与妊娠期高血压的发病有关,并参与了子痫前期病理生理过程。
Objective To investigate the alteration of serum vascular endothelial growth factor and soluble fins-like tyrosine kinase receptor 1（sFlt-1） in patients with preeclampsia. And the correlation of the placental bed spiral arteries and the role role of sFlt-1 and VEGF preeclampisa pathophysiology. Methods Serum vascular endothelial growth factor and soluble fms - like t^sine kinase receptor 1 in peripheral venous blood were determined by ELISA in patients with preeclampsia, severe preeclampsia and healthy controls. Accosting to the Robertson＇ s methods, the placental bed was obtained by biopsies. The physiological changes of spiral arteries were comparatively studied with light microscope. Results Patients with preeclampsia had higher level of serum sFlt-1 and lower serum level of VEGF than that in control group（ P 〈 0.01 ） . There were no significant differences between normal pregnanceis and preeclampsia patients in physiological changes of deciduas segments of spiral arteries （ P 〉 0.05）. The physiological changes of myometrium spiral arteries in preeclampsia were markedly less than that in normal pregnanoy （ P 〈 0.05）. Conclusion The happening of preeclampsia was related closely to the decrease of physiological changes in myometrium spiral arteries of placental bed. Elevated of serum sFlt-1 and decreased VEGF as well as the alteration of the placental bed spiral arteries may be contributed to the pathogenesis of preeclampsia.
出处
《河北医药》
CAS
2008年第5期586-587,共2页
Hebei Medical Journal
