摘要
目的探讨慢性阻塞性肺疾病(COPD)患者白细胞介素-8(IL-8)及白细胞三烯B4(LTB4)水平的变化及在发病中的作用。方法采用酶联免疫吸附法(ELISA)测定30例COPD患者急性加重期和缓解期及20例健康成人的血清及疾液中IL-8及LTB4含量。结果COPD患者急性加重期血清及痰液中IL-8含量分别为(181.41±18.49)ng/L和(74.03±20.64)ng/L,高于缓解期[(152.33±17.48)ng/L和(42.30±14.99)ng/L,均P〈0.05],缓解期显著高于健康对照组(52.75±13.79)ng/L和(17.15±5.36)ng/L,均P〈0.05);COPD患者急性加重期痰液LTB4含量[(2300.91±496.01)ng/L],显著高于缓解期[(1884.14±651.37)ng/L,P〈0.05],COPD患者急性加重期血清LTB4含量[(2044.12±309.43)ng/L]与缓解期及对照组比较差异均无统计学意义[(1941.30±301.62)、(1880.77±302.11)ng/L,均P〉0.05]。结论IL-8与LTB4共同参与了COPD气道炎症反应的过程。
Objective To explore the changes of the levels of IL-8, LTB4 and its function in invasion in patients with chronic obstructive pulmonary disease ( COPD), Methods The concentrations of IL-8 and LTB4 in serum and sputum were determined with enzyme linked immunosorbent assay ( ELISA ) in 30 cases of patients with COPD in acute aggravating stage and paracmasis and in 20 cases healthy adults. Blood routines were detected in all obstructs. Results The concentrations of IL-8 in serum and sputum in acute aggravating stage were ( 181.41 ± 18. 49) ng/L and (74.03 ± 20.64)ng/L, significantly higher than those in paracmasis and the reference group( 152.33±17.48 ) ng/L and ( 42.30 ± 14.99 ) ng/L,P 〈 0.05 ) . The concentrations of IL-8 in serum and sputum in paracmasis were significantly higher than those in reference group [ (52.75 ±13.79) pg/ml and (17.15 ± 5.36) ng/L,P 〈 0.05 ]. The concentrations of LTB4 in sputum in acute aggravating stage were ( 2300.91 ± 496.01 ) ng/L, significantly higher than those in paracmasis and the reference group [ ( 1884.14 ± 651.37 ) ng/L, P 〈 0.05 ]. LTB4 level in acute aggravating is not significantly different from those in paracmasis and the reference group [ ( 1941.30 ± 301. 62 ) ng/L and ( 1880.77 ± 302.11 ) ng/L, P 〉 0.05 ). Conclusion Both IL-8 and LTB4 play important roles in the course of inflammatory reaction of air passage of COPD.
出处
《中国综合临床》
北大核心
2008年第6期582-584,共3页
Clinical Medicine of China