白细胞介素-8、白三烯B4与慢性阻塞性肺疾病气道炎症的关系

Functions of interleukin-8 and leukotriene 114 in chronic obstructive pulomonary disease

目的探讨慢性阻塞性肺疾病（COPD）患者白细胞介素-8（IL-8）及白细胞三烯B4（LTB4）水平的变化及在发病中的作用。方法采用酶联免疫吸附法（ELISA）测定30例COPD患者急性加重期和缓解期及20例健康成人的血清及疾液中IL-8及LTB4含量。结果COPD患者急性加重期血清及痰液中IL-8含量分别为（181．41±18．49）ng/L和（74．03±20．64）ng/L，高于缓解期[（152．33±17．48）ng／L和（42．30±14．99）ng/L，均P〈0．05]，缓解期显著高于健康对照组（52．75±13．79）ng／L和（17．15±5．36）ng／L，均P〈0．05）；COPD患者急性加重期痰液LTB4含量[（2300．91±496．01）ng／L]，显著高于缓解期[（1884．14±651．37）ng/L，P〈0．05]，COPD患者急性加重期血清LTB4含量[（2044．12±309．43）ng／L]与缓解期及对照组比较差异均无统计学意义[（1941．30±301．62）、（1880．77±302．11）ng／L，均P〉0．05]。结论IL-8与LTB4共同参与了COPD气道炎症反应的过程。

Objective To explore the changes of the levels of IL-8, LTB4 and its function in invasion in patients with chronic obstructive pulmonary disease （ COPD）, Methods The concentrations of IL-8 and LTB4 in serum and sputum were determined with enzyme linked immunosorbent assay （ ELISA ） in 30 cases of patients with COPD in acute aggravating stage and paracmasis and in 20 cases healthy adults. Blood routines were detected in all obstructs. Results The concentrations of IL-8 in serum and sputum in acute aggravating stage were （ 181.41 ± 18. 49） ng/L and （74.03 ± 20.64）ng/L, significantly higher than those in paracmasis and the reference group（ 152.33±17.48 ） ng/L and （ 42.30 ± 14.99 ） ng/L,P 〈 0.05 ） . The concentrations of IL-8 in serum and sputum in paracmasis were significantly higher than those in reference group [ （52.75 ±13.79） pg/ml and （17.15 ± 5.36） ng/L,P 〈 0.05 ]. The concentrations of LTB4 in sputum in acute aggravating stage were （ 2300.91 ± 496.01 ） ng/L, significantly higher than those in paracmasis and the reference group [ （ 1884.14 ± 651.37 ） ng/L, P 〈 0.05 ]. LTB4 level in acute aggravating is not significantly different from those in paracmasis and the reference group [ （ 1941.30 ± 301. 62 ） ng/L and （ 1880.77 ± 302.11 ） ng/L, P 〉 0.05 ）. Conclusion Both IL-8 and LTB4 play important roles in the course of inflammatory reaction of air passage of COPD.