摘要
目的:研究并建立灯台叶颗粒中有效成分鸭脚树叶碱含量分析的HPLC方法。方法:系统比较检测波长、流动相、提取方法等因素对灯台叶颗粒中鸭脚树叶碱含量测定的影响,确定最佳分析条件。结果:建立了符合技术要求的HPLC分析方法,色谱柱以十八烷基键合硅胶为填充剂,检测波长287nm,以甲醇-0.01%三乙胺溶液(50∶50)为流动相。结论:该方法分离效果好,灵敏度高,专属性强,能准确、重现的测定灯台叶颗粒中鸭脚树叶碱的含量,可用于灯台叶颗粒产品质量控制。
Objective ： To study and establish the HPLC method for picrinine determination of Dengtai - Ye Granule. Methods ： The influence factors for picrinine determination of Dengtai - Ye Granule by HPLC, such as detecting wavelength, mobile phase, extraction method, et al, have been studied and compared in detail. Results： The HPLC mensuration, up to the requisite technique standard was established. The analysis course used the C - 18 column as the immovable phase and a mixture of methanol- 0. 01% triethylamine solution （50： 50） as the mobile phase. Picrinine was determined with a UV detector at 287nm. Conclusion： The results showed this HPLC method for picrinine determination of Deng Tai - Ye Granule was sensitive, exclusive, accurate and repeatable. It has been used for the quality control of the DengTai - Ye Granule.
出处
《云南中医学院学报》
2008年第3期9-12,共4页
Journal of Yunnan University of Traditional Chinese Medicine
基金
昆明市科技计划项目(昆科计字06S111103)
