水飞蓟宾-磷脂酰胆碱复合物对肝损伤小鼠的保护作用

Protective Effect of Silybin-phosphatidylcholine Compound on Acute Hepatic Injury in Mice

目的探讨水飞蓟宾-磷脂酰胆碱复合物(SPC)对异硫氰酸-α-萘酯(ANIT)所致小鼠急性肝损伤的保护作用。方法40只小鼠随机分成4组,每组10只:正常对照组、ANIT模型组、ANIT+SPC低剂量组(150mg/Kg)、ANIT+SPC高剂量组(300mg/Kg)。前两组给予0.5%羧甲基纤维素钠(CMC-Na)悬液灌胃,后两组分别给予SPC(150、300mg/Kg)CMC-Na悬液灌胃,连续1周。末次给予SPC2h后,正常对照组灌胃给予花生油,其它三组给予ANIT60mg/kg(花生油配制),造成小鼠急性肝损伤模型,16h(禁食不禁水12h)后摘眼球采血,分离血清,检测血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、血清总胆红素(TB)。结果SPC明显降低造模小鼠血清中ALT、AST、TB值,SPC组与模型组比较差异显著(P<0.05)。结论SPC对ANIT造成的急性肝损伤有明显的保护作用。

Objective To observe the protective effect of silybin - phosphatidylcholine compound （SPC） on acute liver damage induced by alpha -naphthylisothiocyanate （ANIT） in mice. Methods Forty mice were randomly and equally divided into four groups： normal group, model group,SPC Ⅰ group, SPC Ⅱgroup. Intragastric administration of 0. 5% CMC -Na suspension were given to the normal and model group, and two quantities of SPC suspension （ 150mg/Kg and 300mg/Kg） were respectively given to SPC Ⅰ and SPC Ⅱgroups for one week. At 12h after last administration of SPC, all of groups besides normal were given ANIT （ dissolved in peanut oil, 60mg/kg） by lavage and the normal group just only were given peanut oil by the same volume and same way. After 16h of ANIT administration, the levels of serum alanine transferase （ ALT）, aspartate aminotransferase （ AST）, serum total bilirubin （TB） were detected by biochemical method. Results SPC could obviously decrease the heightened activities of serum ALT, AST and TB induced by ANIT（ P 〈 0. 05）. Conclusion SPC possess a protective role on ANIT- induced hepatie damage in mice.