三个牦牛品种生长激素受体(GHR)基因PCR-SSCP分析

PCR-SSCP Analysis on the Growth Hormone Receptor Gene in Yak

为了加速牦牛群体的复壮,本实验利用PCR-SSCP技术对甘南、天祝、青海高原3个牦牛品种的生长激素受体基因进行了多态性分析。结果表明:第1、3对引物扩增无多态,第2对引物扩增3个牦牛品种均发现2个等位基因A和B,天祝白牦牛A的基因频率为0.3682,B的基因频率为0.6318;甘南牦牛A的基因频率为0.1596,B的基因频率为0.8404;高原牦牛A的基因频率为0.0700,B的基因频率为0.9300。青海高原牦牛群体处于哈代-温伯格平衡状态,而甘南和天祝牦牛处于不平衡状态,应加强对该基因位点的选择强度。

For the rejuvenescence of yak, we applied PCR-SSCP to analyze the polymorphisms of GHR gene in Gannan yak, Tianzhu white yak and Qinghai plateau yak. The results showed that two allele genes A, B were detected in GHR gene in Gannan yak,Tianzhu white yak and Plateau yak at second primer, the first and third primer had not polymorphism. The frequencies of A and B were in Tianzhu white yak 0. 368 2 and 0. 631 8 respectively; The frequencies of A and B in Gannan yak were 0. 159 6 and 0. 840 4 respectively; The frequencies of A and B in Qinghai platean yak were 0. 070 0 and 0. 930 0 respectively. The population of Qinghai plateau yak was at Hardy-Weinberg equilibrium in the lucus,Tianzhu yak and Gannan yak were not at Hardy-Weinberg equilibrium. We can reinforce to select this gene in the breeding of yak.