LRP和ERCC-1在非小细胞肺癌中的表达及其临床相关性研究

Expression and significance of ERCC-1 and LRP in non-small cell lung carcinoma

目的探讨肺耐药蛋白基因(LRP)和核苷酸酸切除修复交叉互补基因(ERCC-1)在非小细胞肺癌(NSCLC)中的表达及其临床相关性。方法用免疫组化SP法对46例NSCLC组织和16例正常肺组织中两种基因的表达进行检测。结果NSCLC患者ERCC-1和LRP表达阳性在NSCLC组织中别为54.3%(25/46)和82.6%(38/46),在正常肺组织中分别为6.25%(1/16)和6.25%(1/16),ERCC-1表达在肺腺癌表达率为64.2%,腺鳞癌为55.6%,肺鳞癌为47.8%,肺腺癌高于肺鳞癌。LRP表达与组织学分级有关(P<0.05)。结论LRP和ERCC-1在NSCLC组织中的表达率明显高于正常肺组织中表达;LRP表达与患者的组织学分级有关,低分化高表达,高分化低表达;LRP表达阳性的NSCLC患者生存期短,预后差。

Objective To investigate the effect of ERCC-1 and LRP genes in the non-small cell lung carcinoma （NSCLC） and its clinical relevance. Methods The expression of ERCC-1 and LRP genes in 46 patients with NSCLC and 16 patients in normal lung tissue were studied by immanohistochemical SP methods. Results The positive expression rate of ERCC-1 and LRP in NSCLC specimens was 54.3% and 82.6% ,respectively. Positive rate of ERCC-1 and LRP expression in normal lung tissue was 6.25% and 6.25% ,respectively. Expression rates of ERCC-1 in pulmonary adenocarcinoma and squamous adenocarcinoma were 64.2% and 55.6%, respectively, and were significantly higher than that in squamous carcinoma （47.8%）. The LRP expression was singnificantly correlated with dregree of tumor differentiation （P 〈0.05）. Conclusion The positive rate of ERCC-1 and LRP expression in NSCLC tissue was higher than that in normal lung tissue. The LRP expression was correlated with dregree of tumor differentiation. Survival period of patients with LRP positive was shorter than that with LRP negative.