鲮原种群体的D-loop序列分析

Polymorphism study of the mitochondrial DNA D-loop sequences of original population of mud carp

设计了1对可从鲮总DNA中扩增出线粒体D-loop区的引物,获得了937bp的鲮D-loop全序列。应用该对引物对鲮原种群体(子群体h11个样本,子群体q10个样本)进行PCR扩增,测序获得了5′端750bp的有效序列。21个个体共检测到15种单倍型,29个变异位点(4%)。遗传多样性分析发现,原种群体的序列差异为1.0%,子群体h与子群体q各自的序列差异为1.2%和0.9%。UPGMA分子系统树显示,用于研究的21个样本相互混杂,子群体h与子群体q并未出现独立的分支,表明该批鲮原种的子群体h和子群体q并非2个相互独立的母系来源。

The complete mitochondrial D-loop fragment（937 bp total） of mud carp were successfully amplified by a pair of new designed primers.Seven hundreds and fifty bp mitochondrial D-loop fragment of 21 individuals（11 from sub-stock h,10 from sub-stock q） of original population of mud carp were obtained after sequencing and alignment.Fifteen haplotypes were found in the 21 individuals.Twenty-nine nucleotide sites（4%） were variable.The genetic distance within the original population was 1.0%.The genetic distances were 1.2% and 0.9% within samples of sub-stock h and sub-stock q,respectively.In molecular phylogenetic tree constructed by UPGMA method,the 21 samples of the two sub-stocks mixed each other.Although phenotype vairiation existed between sub-stock h and sub-stock q,the two sub-stocks did not show special branches independently.So we believed that the two sub-stocks did not come from two independent maternal origins.