HBV Pre S1蛋白质在酵母细胞中具有转录激活功能

TRANSCRIPTIONAL ACTIVATION BY HBV PRE S1 PROTEIN IN YEASTR

为探讨应用双杂交体系统克隆与HBVPreS1蛋白质结合的肝细胞受体的可行性，我们构建了HBVPreS1蛋白质与酵母GAL4DNAbindingdomain融合蛋白质酵母表达质粒。通过应用该质粒转化酵母菌株SFY526，检测报道基因产物五半乳糖苷酶活性，结果表明HBVPreS1蛋白质在酵母细胞中具有转录激活功能。能否去除PreS1蛋白质转录激活功能区域但却保留其与肝细胞受体的结合区域用于酵母双杂交体系统筛选肝细胞cDNA文库、克隆与PreS1蛋白质结合的肝细胞受体尚需进一步实验探讨。

To explore the feasibility of cloning hepatocyte receptor interacting with HBV Pre S1 protein by two-hybrid system, we made a construct by inserting the HBV pre S1 sequence into a yeastexpression vector, PGBT9, resulting in fusion ofHBV Pre S1 protein with DNA binding dornain of GAL4. By transforming yeast reporter strain SFY526 witb the construct and detecting the rePorter Rene product β-galactosidase activity, the results indicate that the HBV Pre S1 protein activatestranscription in yeast. Whether it is possible to remove the transcriptional activation domain and to retain the hepatocyte receptor interacting dornain of Pre S1 protein for the screening of hepatocyte cDNA library and cloning hepatocyte receptor interactingwith HBV Pre S1 protein needs further investigation.