摘要
雀石蕊经三氯甲烷抽提除去色素，用热水提取，乙醇沉淀，经重结晶微晶纤维素柱层析纯化，得CLS多糖。经SephadexG－150层析为均一性多糖，糖的含量为81．2％。气相色谱检测由鼠李糖（Rha）、阿拉伯糖（Ara）、木糖（Xyl）、甘露糖（Man）、葡萄糖（Glc）和半乳糖（Gal）组成，摩尔比为1∶0．71∶0．45∶4．68∶9．94∶3．64，平均分子量为1．3×106。经高碘酸氧化，Smith降解主要由1→2和1→6甙键连结而成的杂多糖。红外光谱分析具有多糖的特征吸收。
CLS Polysacchride was isolated andpurified from water extract of Cladonia stellaris(Opiz.) Pouzar et Vezda Syn.: Cladonia stellaris(L. ) Raoh. by Sephadex G-150 column chromatogrophy, CLS was to be single substances. Sugar content was 81. 2%. By gas chromatogrophy analysis, CLS was composed of Rha, Ara, Xyl, Man,Clc and Gal. Their molar ratio was about 1: 0. 71:0. 45: 4. 68: 9. 94: 3. 64. The mean molecularweight was estimated to be 1. 3 ×106. Periodate oxidation and Smith degradation were shown to be themain chainis composed of 1→2 and 1→6 linkage.The IR showed the characteristic absorption ofpolysaccharide.
出处
《西安医科大学学报》
CSCD
1997年第4期476-478,共3页
Journal of Xi'an Medical University(Chinese)
关键词
省石蕊
地衣
多糖
药用
cladonia stellaris
lichenes
polysaccharide
