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丙戊酸钠对人宫颈癌细胞增殖和细胞周期的影响 被引量：1

Impact of Sodium Valproate on Cell Proliferation and Cell Cycling in Human Hela Cells
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摘要 [目的]研究组蛋白去乙酰化酶抑制剂丙戊酸钠对人宫颈癌Hela和Siha细胞株增殖和细胞周期的影响。[方法]用不同浓度丙戊酸钠分别作用Hela和Siha细胞,MTT法测生长抑制率,流式细胞仪检测细胞周期,RT-PCR和Westernblot检测p21WAF1/CIP1mRNA和蛋白改变。[结果]丙戊酸钠对宫颈癌Hela和Siha细胞生长抑制呈剂量依赖性;丙戊酸钠使Hela和Siha细胞阻滞于G0/G1期;丙戊酸钠上调p21WAF1/CIP1mRNA和蛋白表达。[结论]丙戊酸钠能上调p21WAF1/CIP1表达,促使细胞阻滞于G0/G1期,从而抑制宫颈癌Hela和Siha细胞生长。
作者 李娜 于世英
机构地区 华中科技大学同济医学院附属同济医院
出处 《中国肿瘤》 CAS 2008年第6期502-504,共3页 China Cancer
基金 湖北省科技攻关计划(2005AA304B07)
关键词 丙戊酸钠 P21^WAF1/CIP1 细胞周期 宫颈肿瘤
分类号 R730.23 [医药卫生—肿瘤]
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参考文献10

  • 1Gurvieh N, Tsygankova OM, Meinkoth JL, et al. Histone deacetylase is a target of valproie acid-mediated cellular differentiation[J]. Cancer Res, 2004, 64(3): 1079-1086.
  • 2Anton M, Horky M, Kuchtickova S, et al. Immunohistochemical detection of acetylation and phosphorylation of histone H3 in cervical smears[J]. Ceska Gynekol, 2004, 69 (1):3-6.
  • 3Chavez-Blanco A, Segura-Pacheco B, Perez-Cardenas E, et al, Histone acetylation and histone deacetylase activity of magnesium valproate in tumor and peripheral blood of patients with cervical cancer. A phase Ⅰ study [J]. Mol Cancer, 2005, 4(1):22.
  • 4Kaiser M, Zavrski I, Sterz J, et al. The effects of the histone deacetylase inhibitor valproic acid on cell cycle, growth suppression and apoptosis in multiple myeloma [J]. Haematologica, 2006, 91 (2):248-251,
  • 5Takai N, Desmond JC, Kumagai T, et al. Histone deacetylase inhibitors have a profound antigrowth activity in endometrial cancer cells[J]. Clin Cancer Res, 2004, 10(3): 1141-1149.
  • 6Hrzenjak A, Moinfar F, Kremser ML, et al. Valproate inhibition of histone deacetylase 2 affects differentiation and decreases proliferation of endometrial stromal sarcoma cells[J]. Mol Cancer Ther, 2006, 5 (9):2203-2210.
  • 7Armeanu S, Pathil A, Venturelli S, et al. Apoptosis on hepatoma cells but not on primary hepatoeytes by histone deaeetylase inhibitors valproate and ITF2357[J]. J Hepatol, 2005, 42(2):210-217.
  • 8Shabbeer S, Kortenhorst MS, Kachhap S, et al. Multiple Molecular pathways explain the anti-proliferative effect of valproic acid on prostate cancer cells in vitro and in vivo [J]. Prostate, 2007, 67(10):1099-1110.
  • 9Huang X, Guo B. Adenomatous polyposis coli determines sensitivity to histone deacetylase inhibitor-induced apoptosis in colon cancer cells[J]. Cancer Res, 2006, 66(18): 9245-9251.
  • 10Richon VM, SandhoffTW, Rifkind RA, et al. Histone deacetylase inhibitor selectively induces p21WAF1 expression and gene-associated histone acetylation [J]. Proc Natl Acad Sci U S A, 2000, 97(18):10014-10019.

同被引文献18

  • 1Batty N, Malouf GG, Issa JP, et al. Histone deacetylase in- hibitors as anti-neoplastic agents [J]. Cancer Lett, 2009, 280 (2) :192.
  • 2Sawan C,Herceg Z. Histone modifications and cancer[J]. Adv Genet,2010,70:57.
  • 3Johnstone RW. Histone-deacetylase inhibitors: novel drugs for the treatment of cancer [J]. Nat Rev Drug Discov, 2002,1:287.
  • 4Children's Oncology Group, Fouladi M, Furman WL, et al. Phase I study of depsipeptide in pediatric patients with re- fractory solid tumors:a Children "s Oncology Group report [J]. J Clin Onco:,2006 ,24 :3678.
  • 5Anton M, Horky M, Kucht:ckov6 S, et al. Immunohisto- chemical detection of acetylation and phosphorylation of histone H3 in cervical smears [J]. Ceska Gynekol, 2004, 69(1) :3.
  • 6Yugawa T, Kiyomo T. Molecular mechanisms of cervical c- arcinogenesis by high-risk human papilloma viruses:novel function of E6 and E7 oncoproteins[J]. Rev Med Virol, 2009,19 (2) :97.
  • 7Phiel CJ, Zhang F, Huang EY, et al. Histone deacetylase is a direct target of valproic acid, a potent anticon-vulsant, mood stabilizer, and teratogen[J]J Biol Chem, 2001,276 (39) :36734.
  • 8Gottlieher M, Minueci S ,Zhu P, et al. Valproic acid defines a novel class of HDAC inhibitors inducing differentiation of transformed cells [ J ]. EMBO,2001,20 ( 24 ) :6969.
  • 9Armeanu S, Pathil A, Venturelli S, et al. Apoptosis on hepa- toma cells but not on Primary hepatocytes by histone deacetylase inhibitors valproate and ITF2357 [ J ]. J Hepa- tol,2005,42(2) :210.
  • 10Kaiser M, Zavrski I, Sterz J, et al. The effects of the histone deacetylase inhibitor valproic acid on cell cycle, growth sup- pression and apoptosis in multiple myeloma[J]. Haemato- logica,2006,91 (2) :248.

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中国肿瘤
2008年 第6期

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