RalB在多种肿瘤细胞中的表达及意义

Expression and Significance of V-ral Simian Leukemia Viral Oncogene Homolog B in Different Tumor Cell Lines

目的探讨RalB(v-ral simian leukemia viral oncogene homolog B,ras related;GTP binding protein)蛋白在多种肿瘤细胞中的表达及意义。方法以人胚肾293T细胞为正常细胞对照,选用8种不同组织的肿瘤细胞,采用抗RalB的单克隆抗体通过Western blot方法检测其RalB蛋白的表达。结果RalB在293T细胞中表达明显降低,在乳腺癌细胞株MCF-7、卵巢癌细胞株HO8910、宫颈癌细胞株Hela、肺癌细胞株、结肠癌细胞株LOVO、SW480中均有表达,其中RalB蛋白在结肠癌细胞株SW480中表达明显增高。结论RalB可能以组织特异性的方式在不同肿瘤细胞中表达。

Objective To investigate the expression and meaning of RalB in different tumor cells. Methods The monoclonal antibody against RalB was used to identify the expression of RalB in eight kinds of tumor cells by Western-Blot. Human embryo kidney cell line was used as normal cell control. Results RalB expression was observed in the cell lines of MCF-7 breast carcinoma,ovarian cancer HO8910 cell line, cervical, cancer Hela cells,pulmonary carcinoma and colon carcinoma LOVO and SW480 cells,with considerably high expression level in SW480 cells. Condusions RalB may express in different tumor tissues in tissue-specific manner.