应用高密度单核苷酸多态性芯片分析胰腺癌细胞基因缺失

Analysis of Homozygous Deletion in Human Pancreatic Cancer by High-resolution Single-nucleotide Polymorphism Array

目的探索胰腺癌癌变过程中的基因异常,研究胰腺癌基因组的纯合性缺失。方法应用高密度单核苷酸多态性芯片,检测17种胰腺癌细胞株基因组的纯合性缺失,并利用分析软件进行数据分析,用PCR验证纯合性缺失位点。结果17种胰腺癌细胞基因组中,发现70个区域纯合性缺失,其中28个区域无已知基因,42个区域有基因。35个区域经过PCR验证,29个区域经证实为纯合性缺失,芯片的准确度为82.9%,其中最小缺失区域为6kb。结论应用高解析度的SNP基因芯片可以检测胰腺癌全基因组范围内的精细的纯合性缺失位点。

Objective To analyze homozygous deletions in pancreatic cancer so as to chart the molecular genetic events in pancreatic cancer. Methods Genome-wide homozygous deletions in 17 established pancreatic cancer cell hnes were analyzed using high-density single nucleotide polymorphism （SNP） army. The datas were analyzed by software, and the results were verified by PCR. Results 70 homozygous deletions were detected in at least one cell line, including 28 regions without known genes and 42 regions with known genes. 35 regions were tested by PCR and 29 regions were verified with minimal size of 6 kb. The accuracy of the chip was 82.9%. Conclusion Genome-wide homozygous deletion can be detected by using high-resolution SNP arrays and the narrow alterations can be picked up. The novel candidate genes identified might become targets for the early diagnosis and therapy of pancreatic cancer.