摘要
目的通过检测三氧化二砷(As2O3)对肝癌SMMC-7721细胞周期素依赖性激酶抑制剂(CDKI)p27kip1及S期激酶相关蛋白2(Skp2)的影响,探讨其抗癌作用机制。方法体外培养人肝癌细胞株SMMC-7721,用2μmol/LAs2O3处理72h,流式细胞仪检测细胞周期变化及细胞凋亡率,应用Westernblot方法检测p27kip1、Skp2及CDK2基因编码蛋白的表达。结果与对照组比较,As2O3使SMMC-7721细胞周期阻滞在G2/M期。经As2O3作用的人肝癌细胞p27kip1基因编码蛋白表达增加,而p27kip1相关分子Skp2及CDK2的表达减少。结论As2O3可干扰细胞周期的进程,抑制SMMC-7721细胞的增殖。其作用机制与上调p27kip1蛋白及下调Skp2、CDK2有关。
Objective To investigate the modulation of CDKI p27^kip1 and p27^kip1-related protein-Skp2 of arsenic trioxide（As2O3）in human hepatomaeellular eareinoma（HCC） SMMC-7721eells,offering experimental evidences for clinical application of As2O3 in HCC. Methods Cultured in vitro,HCC SMMC-7721 were treated for 72 h with 2 μmol/L arsenic trioxide,The cell cycle and apoptosis index were detected by flow cytometry（FCM）,Western blot was used to detect protein expression of p27^kip1,Skp2,CDK2. Results SMMC-7721 cell cycle was arrested in G2/M, compared with the control,arsenic trioxide induced increase of the expression of p27^kipl and decreased levels of Skp2 and CDK2 in HCC. Conclusion Arsenic trioxide could disturb cell cycle progression of SMMC-7721 cell. The mechanksm may relate to that arsenic trioxide up-regulate expression of p27^kip1 and down-regulate expression of Skp2 and CDK2.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2008年第2期191-193,206,共4页
Suzhou University Journal of Medical Science
