脑胶质瘤组织中Per1和Per2的表达及临床意义

Expression and clinical significance of Per1 and Per2 in gliomas

目的探讨生物钟基因Per1和Per2在人脑胶质瘤细胞和周围正常脑组织中的表达特征。方法采用免疫组化方法检测33例在不同级别的脑胶质瘤和周围正常组织中,生物钟基因Per1和Per2蛋白的表达。结果Per1和Per2在正常脑组织中阳性表达率均为100%;Per1在胶质瘤组织中阳性表达率为84.85%(28/33),Per2在胶质瘤组织中阳性表达率为69.70%(23/33);Per1在胶质瘤Ⅰ-Ⅱ级表达强度高于Ⅲ-Ⅳ级,Per2在胶质瘤Ⅰ-Ⅱ级与Ⅲ-Ⅳ级的表达强度无明显差异。结论生物钟基因Per1和Per2在胶质瘤与正常脑组织存在着阳性表达,Per1表达强度与胶质瘤的恶性程度呈负相关系。

Objective To investigate expression of Perl and Per2 in normal brain and gliomas. Methods Expression of Perl and Per2 was determined in normal brain and gliomas by using immunohistochemical technique. Results The expression of Perl and Per2 were significantly observed in normal brain tissiues. Expression of Perl in gliomas is 84. 85% （28/33） ;expression of Per2 in gliomas is 69.70% （28/33）. Expression level of Perl in the levelⅠ to Ⅱis higher than that in the level Ⅲ to Ⅳ, and there was no significant different. Conclusions Expression levels of Perl and Per2 in the glomas and in normal brain tissues can be observed,and expression levels of Perl will decrease when the malignancy of the gliomas is to rise.