脑缺血再灌流损伤实验研究

EXPERIMENTAL STUDY ON INJURIES AFTER CEREBROVASCULAR REPERFUSION

目的：探讨低温、ATP、醒脑净对脑缺血再灌流损伤的保护作用。方法：选纯种白兔通过“四管闭塞法”制成兔全脑缺血再灌流损伤模型，分四组测定丙二醛（MDA）和谷胱甘肽过氧化物酶（GSH－PX），同时电镜观察脑组织切片。结果：再灌流30min后实验各组与对照组相比，低温组和ATP组MDA显著低于对照组（P＜0．01），醒脑静组MDA显著低于对照组（P＜0．05）；低温组和ATP组GSH－Px显著高于对照组（P＜0．01），醒脑静组GSH－Px显著高于对照组（P＜0．05）。其中低温组的32～34℃组与26～28℃组之间MDA和GSH－Px无显著差别（P＞0．05）。电镜观察显示实验各组的脑损伤比对照组轻。结论：低温、ATP、醒脑净对脑缺血再灌流损伤有保护作用。

Aim:To evaluate the efficacy of four protective treatments to the brain injuries of cerebrovascular reperfusion in rabbit. Methods: Rabbit experimental models were prepared by the method of 'four arteries occlusion'. Animals were treated by reperfusion with moderate hypothermia, or mild hypothermia, or intravenous injection of ATP and Xing Nao Jing (a Chinese traditional medicine) respectively for 30 minutes (each group has 6 rabbits). Plasmic MDA and GSH-px were measured to estimate the degree of brain injuries. Sections of brain tissue were also studied by electron microscope (EM). Results:The concentration of plasmic MDA in each group treated with protective methods were significantly lower than that in the control (P<0.05) after 30 min reperfusion,meanwhile, the concentrations of plasmic GSH-Px in each group treated with protective methods were significantly higher than that in the control (P<0.05). The study of brain tissue's sections by EM also showed less injuries in each group treated with protective methods. Conclusion: Hypothermia, intravenous injection of ATP and Xing Nao Jing all have the efficacy that protects the brain tissue from jnjuries by cerebrovascular reperfusion. There is no significant difference between groups treated with moderate and mild hypothermia.