大鼠左侧MCA阻塞后运动和行为的观察

Observation of motor and behavior in rats blocked the left middle cerebral artery

目的探讨大鼠一侧MCA阻塞后行为改变的特征和评定其运动障碍的客观方法．方法雄性Wistar大鼠分成假手术和阻塞两组，阻塞组用直径0.2mm的尼龙线经在颈内动脉插入到大脑前动脉，阻塞大脑中动脉（MCA）根部。术后1、2、4、8，24、48h观察神经行为。用握－引和网屏测验评定肌力，转棒测验测定运动协调能力。结果典型的偏瘫4h后难以用肉眼查出，转圈有时相性。提尾悬空试验阳性体征持续到术后3d运动协调障碍持续到本后7d．结论提尾悬空试验阳性是一侧MCA阻塞的特征性表现，转体测验能对运动协调能力定量评定．

To probe into behavior characteristics in rats blocked the left middle cerebral artery and it's objetive method of evaluate motor.Method Male Wistar rats were devided into sham operated and blocked group. Rats in the blocked group were inserted a nylon thread with a diameter of O.2rnm into the anterior cerebral artery through the internal carotid artery,blocking the root of the left middle cerebral artery. Behavior were observed at 1,2,4,8,24,48 hours after peration. Muscle strength was evaluated by prehensile-traction and screen tests. Capability of motor coordination was tested by rotarod performance tests.Results Typical hemiplegia was diffcult to detected nakedly 4 hours after operation, The circle was varied with the time phase. The positive sign of suspended body by holding the tail persisted for 3 days, abnormal motor coordination persistod for 7days.Conclution The positive sign of suspended body holding the tail was characteristics in rats blocked a middle cerebral artery. Motor coordination cou1d be quantitativelly evaluated by rotarod performance test.