脾虚型慢性乙型肝炎患者树突细胞功能的变化

Changes of Function of Dendritic Cells in Patients with Chronic Hepatitis B of Spleen Deficiency

目的探讨脾虚型慢性乙型肝炎患者树突状细胞(DC)的功能变化。方法分离培养慢性乙型肝炎脾虚、非脾虚患者及健康人外周血DC,以倒置显微镜和电镜观察DC外部形态和超微结构,流式细胞仪测定DC表面HLA-DR、CD86、CD80、CD40、CD14、CD11c的表达,混合淋巴细胞反应测定两组DC的功能。结果慢性乙型肝炎脾虚组DC表面的协同刺激分子HLA-DR、CD86、CD80、CD40、CD11c均较正常对照组明显降低(P<0.01);与非脾虚组相比,脾虚组HLA-DR、CD80、CD11c降低明显(P<0.05)。两组DC的刺激活性均降低,且脾虚组比非脾虚组降低更明显(P<0.05)。结论慢性乙型肝炎脾虚患者的DC成熟度降低,功能缺陷,提示脾虚与DC成熟障碍具有明显的相关性。

Objective To research the function of dendritic cells （DC） in chronic HBV infectors of spleen deficiency. Methods The peripheral blood mononuclear cells （PBMC） in chronic HBV infectors of spleen deficiency, or of non-spleen deficiency, or in healthy people were isolated and cultured. The shape and ultrastructure of DC were investigated by taking photographs with invert microscope and electron microscope. The surface marks expressions of HLA-DR, CD86, CD80, CD40, CD14 and CDllc were detected by flow cytometry, and the function of DC were assayed by mixed lymphocyte reaction（MLR）. Results The levels of HLA-DR, CD86, CD80, CD40 and CDllc on DC surface were decreased significantly in spleen-deficiency chronic HBV infectors （P〈 0.01）; the levels of HLA-DR, CD80 and CDllc in spleen deficiency group were lower than in spleen non-deficiency group （P〈 0.01, P〈 0.05）. The stimulating capability of DC was all degraded in two groups （P 〈 0.05）. Conclusion The degree of maturity of DC among chronic HBV infectors of spleen deficiency was degraded with functional deficiency, revealing a correlativity between spleen deficiency and DC maturity.