端粒酶反转录酶基因在肝细胞癌中的表达及其意义

Expression of hTERT Gene in Hepatocellular Garcinoma and Its Clinical Significance

目的研究肝细胞癌中端粒酶反转录酶（human telomerase reverse transcriptase,hTERT）基因的表达及其临床意义。方法采用原位杂交方法分别对肝细胞癌及肝炎后肝硬化组织中hTERTmRNA进行检测。结果肝癌中hTERTmRNA表达阳性率明显高于肝硬化组织,差别有统计学意义（P〈0.01）;中、低分化的肝癌hTERT mRNA阳性强度高于高分化者,差别有统计学意义（P〈0.05）;肝癌中hTERTmRNA表达阴性组患者术后3年生存率高于阳性组患者,差别有统计学意义（P〈0.05）;hTERTmRNA表达弱阳性的肝癌患者生存时间较强阳性者延长,差别有统计学意义（P〈0.05）。结论hTERTmRNA阳性表达普遍存在于肝癌组织中,通过检测肝癌中hTERTmRNA表达情况可用来作为肝癌诊断及预后估计的新标志物。

Objective To investigate the expression of human telomerase reverse transcriptase （hTERT） gene in hepatocellular carcinoma and its clinical significance.Methods The hTERTmRNA was detected by situ hybridization in 37 cases of hepatocellular carcinoma （HCC） and 33 cases of liver cirrhosis.Results The positivity rate of hTERTmRNA expression in HCC was much higher than that in liver cirrhosis（P〈0.01）.The positive extent of hTERT gene expression in medium and lower HCC cell differentiation was higher than that in higher HCC cell differentiation （P〈0.05）.The 3-year survival rate post-operation was higher in hTERTmRNA-negtivity group of HCC than that in hTERTmRNA-positivity group of HCC（P〈0.05）.The patients with weak positivity of hTERTmRNA expression in HCC lived much longer than those with strong positivity（P〈0.05）.Conclusion The positive expression of hTERTmRNA is most common in HCC tissue.The hTERTmRNA may be a new biomarker for prognosis and early diagnosis of HCC.