激活素A及其ⅡA型受体在小鼠肝损伤模型肝组织中的表达

Expressions of activin A and its type ⅡA receptor in liver injury of model mice induced by Con A

目的:探讨激活素A及其ⅡA型受体在Con A诱导的小鼠肝损伤模型肝组织中的表达形式及其可能的作用。方法:小鼠尾静脉注射Con A(10 mg.kg-1),每周1次,连续4周,建立小鼠肝损伤模型(n=24),另设正常对照组(n=24),于末次注射后的24、72、120及168 h,各组(n=6)分批检测小鼠血清酶变化及肝脏组织病理损伤程度,同时采用荧光定量RT-PCR法检测激活素A及其ⅡA型受体的表达水平。结果:在末次注射后72 h肝组织病理损伤最为严重,肝小叶结构紊乱,肝细胞索消失,细胞肿胀,呈气球样变,以后逐渐恢复;激活素A蛋白水平及其mRNA表达与其ⅡA型受体mRNA表达呈平行状态,于注射后72 h达高峰,与对照组比较差异具有显著性(P<0.05)。结论:激活素A与其ⅡA受体变化与肝组织病理损伤呈平行状态,提示激活素A可能参与了Con A诱导肝损伤模型小鼠的肝损伤。

Objective To investigate the expression pattern of activin and type Ⅱ A receptor of activin （ActR Ⅱ A） and its possible effect on liver injury of Con A-induced model mice. Methods 24 mice were injected with Con A （10 mg·kg^-1） in the tail vein weekly for 4 consecutive weeks to cause liver injury and normal control mice （n=24） were used as control. Then the levels of serum ALT and AST and severity of liver injury were examined, as well as the expression levels of activin and ActR Ⅱ A mRNA in liver were detected by using fluorescent quantitive PCR. Results The liver injury was most obvious at 72 h after the last injection, such as structure of liver lobules disordered, hepatic cord disappeared, balloon-like hepatocytes swelled, and then gradually repaired from 120 h to 168 h. The activin A protein level and its mRNA expression paralleled with ActR Ⅱ A mRNA expression, and also reached the peak at 72 h after the last injection, which had significant difference compared with control mice （P〈0.05）. Conclusion The changes of activin A and ActR Ⅱ A are positively correlated with the severity of liver pathological changes, which indicate that activin may take part in Con A-induced mouse liver injury.