黄芩总黄酮对S180、Hep-A-22和Bcap-37肿瘤细胞的抑制作用

Inhibitory effects of total flavonoids of scutellaria baicalensis georgi on S_(180),Hep-A-22 and Bcap-37 tumor cells

目的:研究黄芩总黄酮对S180、Hep-A-22和Bcap-37肿瘤细胞增殖及S180和Hep-A-22荷瘤小鼠肿瘤生长的抑制作用。方法:将S180、Hep-A-22和Bcap-37肿瘤细胞分别加入终浓度为12.5、25.0、50.0及100.0 mg.L-1的黄芩总黄酮,采用四氮唑盐衍生物(XTT)、MTT法测定细胞增殖抑制率;将S180和Hep-A-22荷瘤小鼠分为空白对照组、环磷酰胺(CTX)阳性药对照组(30 mg.kg-1,隔日给药)、黄芩总黄酮大(200 mg.kg-1.d-1)、中(100 mg.kg-1.d-1)和小剂量组(50 mg.kg-1.d-1),连续给药15 d后剥离肿瘤,称取瘤重,计算其肿瘤生长的抑制率。观察各给药组Hep-A-22荷瘤小鼠连续用药10 d后生命延长率。结果:黄芩总黄酮对S180、Hep-A-22和Bcap-37肿瘤细胞的抑制率随给药浓度的增加而显著升高,其IC50值分别为16.04、17.74和9.05 mg.L-1;与空白对照组比较,黄芩总黄酮大、中、小剂量组S180、Hep-A-22荷瘤小鼠的肿瘤重量均明显低于空白对照组(P<0.05或P<0.01),随给药剂量的增加,其肿瘤抑制率增加(P<0.05或P<0.01)。黄芩总黄酮大、中、小剂量组的Hep-A-22荷瘤小鼠的平均生存天数均显著高于空白对照组(P<0.01),其生命延长率较空白对照组显著增加(P<0.01)。结论:黄芩总黄酮对S180、Hep-A-22和Bcap-37肿瘤细胞的增殖及S180和Hep-A-22荷瘤小鼠肿瘤生长均具有抑制作用。

Objective To study the inhibitory effects of total flavonoids of scutellaria baicalensis georgi （TFSB） on Sl80, Hep-A-22 and Bcap-37 tumor cell proliferation in vitro and on Sl80, Hep-A-22 in mice bearing tumor in vivo. Methods In vitro, Sl80, Hep-A-22 and Bcap-37 cells were divided into control group and TFSB groups （12.5, 25.0, 50. 0, 100.0 mg·L^-1） . The inhibitory effects of TFSB on proliferation of Sl80 and Hep-A-22 were measured by XTT colorimetric assay, and Bcap-37 cells were measured by MTT colorimetric assay. In vivo, the mice bearing tumor were divided into control group, CTX group （30 mg·kg^-1）, high, middle, low doses TFSB groups （200, 100, 50 mg·kg^-1） . After the mice bearing Sl80 and Hep-A-22 tumor cells were treated with TFSB for 15 d, the tumor weights were measured, the inhibitory rates of Sl80 and Hep-A-22 were calculated and survival of Hep-A-22 was measured after administration of TFSB for 10 d. Results TFSB inhibited the proliferation of Sl80, Hep-A-22 and Bcap-37 cells, IC50 values were 16.04, 17.74 and 9.05 mg·L^-1, respectively. The tumor weight of mice bearing Sl80 and Hep-A-22 cells in TFSB groups （200, 100, 50mg·kg^-1） were lowered than that in control （ P〈0. 01 or P〈0.05）. The tumor inhibitory rates were increased in dose-dependent manner. The survival of mice hearing Hep-A-22 in TFSB groups were higher than that in control （P 〈 0.01 ）. Conclusion TFSB may possess significantly inhibitory effect on proliferation of Sl80, Hep-A-22 and Bcap-37 cells in vitro and the growth of tumor in mice bearing Sl80 and Hep-A-22 in vivo.