氧化苦参碱对大鼠局灶性脑缺血性损伤的保护作用

Protective effects of oxymatrine on injury induced by focal cerebral ischemia in rats

目的:观察氧化苦参碱对大鼠局灶性脑缺血性损伤的作用,并探讨其作用机制。方法:将大鼠随机分为假手术组(n=8)、脑缺血模型组(n=8)、路路通组(LLT,31.25mg.kg-1,n=8)及OMT35、70和105 mg.kg-13个剂量组(n=8)。结扎大脑中动脉制备大鼠局灶性脑缺血模型,术后腹腔注射给药5 d,以神经学评分及脑梗塞体积为指标观察氧化苦参碱对大鼠局灶性脑缺血性损伤的保护作用;同时测定大鼠血清NO及脑组织中MPO含量以探讨氧化苦参碱的作用机制。结果:与模型组比较,氧化苦参碱35、70和105 mg.kg-1剂量组大鼠脑梗塞体积减少(P<0.05,P<0.01,P<0.001),70和105 mg.kg-1剂量组神经学评分降低(P<0.01);氧化苦参碱各剂量组大鼠血清NO及脑组织中髓过氧化物酶含量降低(P<0.05或P<0.01)。结论:氧化苦参碱对局灶性脑缺血大鼠的脑缺血性损伤具有明显的保护作用,抗炎可能为其作用途径之一。

Objective To explore the effects of oxymatrine （OMT） on injury induced by focal cerebral ischemia in rats and its mechanism. Methods Rats were randomly divided into sham （n=8）, cerebral ischemia model （n=8）, Lulu Tong （LLT,31.25mg·kg^-1,n=8） and OMT 35, 70 and 105 mg·kg^-1 groups （n=8）. The focal cerebral ischemia was induced by middle cerebral artery occlusion in rats. The drugs were administrated by intraperitoneal injection. The neurological score and infarct volume were used to evaluate the protective effects of OMT in focal cerebral ischemia rats. The NO content in serum and myeloperoxidase （MPO） content in brain tissue were determined to study the its mechanisms. Results Compared with model group, the infarct volumes were decreased in OMT 35, 70 and 105 mg·kg^-1 groups （P〈0.05, P〈O. 01, P〈0. 001） and the neurological scores were decreased in OMT 70 and 105 mg·kg^-1groups （P〈0. 01）; The NO content in serum and MPO content in brain tissue in OMT groups were decreased （P〈0.05, P〈0. 01）. Conclusion OMT has protection against injury induced by cerebral ischemia in rats, The anti-inflammation effects may be one of the mechanisms.