摘要
以3个新疆早实核桃品种为试材,对外植体的消毒方法、取材时间、培养基和外植体类型等因子进行了初步研究。结果表明:两次升汞消毒法能增强茎尖的消毒效果;以嫩枝茎段为外植体时,适宜的取材时期为6月,以腋芽饱满半木质化的培养效果较好;适宜的培养基类型是DKW和1/2MS,嫩枝茎尖的培养效果要好于茎段;3个品种的初代培养效果存在差异。
With three Xinjiang precocious walnut varieties as experimental materials, the influence of sterilization ways of explants, time of material - taking; and types of medium and explants were preliminary studied. The results showed that two steps of sterilization with mercuric chloride enhanced the sterilization effect for shoot tip ; the most suitable time for taking tender shoots with full axilla buds and semi - lignification as explants was in June; the shoot tip had better culture results than tender shoots; and that DKW and 1/2MS medium were both fitting for the precocious walnut explants in vitro culture ; differences of the primary culture effect of three varieties was observed.
出处
《塔里木大学学报》
2008年第1期10-12,共3页
Journal of Tarim University
基金
新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放课题(编号:BR0601)
关键词
早实核桃
外植体
初代培养
precocious walnut
explants
primary culture