益气养阴 活血化瘀方对病毒性扩张型心肌病小鼠心肌ICAM-1表达水平的影响

Effect of Yiqi Yangyin Huoxue Huayu on ICAM-1 in Cardic Muscle of Rat Model of Viral Dilated Cardiomyopathy

目的:通过观察益气养阴、活血化瘀方对病毒性扩张型心肌病小鼠心肌ICAM-1表达水平的影响,探讨其治疗病毒性扩张型心肌病的作用机理。方法:210只Balb/c小鼠随机分为4组,空白组30只,模型组、中药低剂量组和中药高剂量组各60只。通过反复多次腹腔注射柯萨奇B3m病毒,建立病毒性扩张型心肌病小鼠模型。中药低剂量组、中药高剂量组用益气养阴、活血化瘀方治疗。于实验终点观测各组小鼠心肌病理形态学改变、死亡率、体重、心脏质量指数变化,SABC法检测小鼠心肌ICAM-1表达水平。结果:中药低剂量组、中药高剂量组小鼠心肌ICAM-1表达水平显著低于模型组(P<0.01),且心肌病理改变亦较模型组轻微。结论:益气养阴、活血化瘀方可下调病毒性扩张型心肌病小鼠心肌ICAM-1表达水平,减轻炎性细胞损伤和自身免疫损伤。

Objective ： To investigate the effect of the prescription of Yiqi Yangyin Huoxue Huayu on the rat model of viral dilated cardiomyopathy by observing the expression of ICAM-1 in cardiac muscle. Methods ： Replicated the animal models of the viral dilated cardiomyopathy with the method of injecting the CVB3m virus to the peritoneal cavity of the Balb/c rats. 210 Balb/c rats were devided into 4 groups at random, ie： blank group, model group, low dose traditional Chinese medicine group and high dose traditional Chinese medicine group. The blank group has 30 rats. The other three group has 60 rats each. Treat the rats of low dose traditional Chinese medicine group and high dose traditional Chinese medicine group with the prescription of Yiqi Yangyin Huoxue Huayu. Observe the change of pathomorphology in the car- diac muscle tissue, the rate of death , the weight change and the cardiac weight index in the end of the experiment. At the same time SABC method was used to exam the expression of ICAM-1 in cardiac muscle. Results ： Compaired with the model group the expression of ICAM-I in cardiac muscle were significantly lower and the change of pathomorphology in the cardiac muscle tissue were lighter in the low dose traditional Chinese medicine group and high dose traditional Chinese medicine group. Conclusion ：The prescription of Yiqi Yangyin Huoxue Huayu can reduce the the expression of ICAM-1 in cardiac muscle and relieve the damage of inflammatory reaction and autoimmunity in DCM.