异鼠李素对ECV304细胞分泌的t-PAPAI-1vWf的影响

The Influence of t-PA PAI-1 and vWf That Isorhamnetin for ECV304 Cell

目的:探讨异鼠李素对ECV304细胞分泌的t-PA PAI-1 vWf的影响。方法:用不同浓度的异鼠李素与ECV304细胞培养18h后,用ELISA法检测培养液中t-PA、PAI-1、vWf的含量。结果:不同浓度的异鼠李素均可抑制内皮细胞PAI-1的分泌,与空白对照组相比,高、中剂量组有显著性差异(P<0.01),低剂量组无显著性差异,并随着异鼠李素浓度的增加PAI-1含量呈剂量依赖性减少;不同浓度异鼠李素对内皮细胞分泌的t-PA和vWf均无明显影响。结论:异鼠李素可能在一定程度上通过降低内皮细胞PAI-1的分泌,影响纤溶系统活性。

Objective ： Discuss the influence of t - PA, PAI- 1 and vWf that different isorhamnetin secretes for ECV304 cell. Method： Use the content of t - PA, PAI- 1 and vWf in ELISA law detection nutrient solution with ECV304 cell and different isorhamnetin of different density after cultivating 18 hours. Result： Different isorhamnetin of density may restrain endothelial cell PAI- 1 secrete, in compared with blank contrast group, it is high to have, dosage group signifi- cance discrepancy （ P 〈 0.01 ） low dosage group to have no significance discrepancy , and along with different isorham- netin density increase PAI- 1 content to submit dosage dependence decreasing; Different density different isorhamnetin the vWf and t - PA that endothelial cell secretes have no obvious influence. Conclusion ： Different isorhamnetin may pass through the expresssion of leather cell PAI- 1 in reduction on certain degree and secretes level, influence is fine dissolve system is active play the part of to fight thrombus role.