一株氯氰菊酯降解菌16S rDNA,gyrB和GyrB的系统发育分析(英文)

Phylogenetic assessment of a new cypermethrin-degrading bacterium Gordonia sp.D2 based on 16S rDNA,gyrB and GyrB sequences

从农药厂污水处理池中分离得到一株氯氰菊酯降解菌,在30℃,pH 7.0的条件下,无机盐培养基中100mg/L的氯氰菊酯,经过7.5天,能够降解大约52.3%,外加碳源能够明显提高其降解性能。生理生化实验结合16S rDNA,gyrB和GyrB的系统发育分析,将其归为Gordonia菌属。在16S rDNA水平上,其与G.amicalis DSM44461和G.hydrophobica DSM44015T的相似值最高,为98.1%;而在gyrB和GyrB水平上,其与G.hydrophobica JCM10086的相似值最高,分别为86.8%和91.1%。通过对所构建的系统发育树进行评估,表明16S rDNA序列适用于将分离菌株鉴定到属的水平上,而gyrB和GyrB更适用于在属内种的水平上进行系统发育的分析。

A cypermethrin - degrading bacterium named as Gordorda sp. D2 was isolated from activated sludge of a wastewater treatment bioreactor. At 30 ℃and pH 7.0, the removal rate of 100 mg cypermethrin L^-1 was approximately 52.3% in mineral salts medium within 7.5 days, and extra carbon source addition could enhance the biedegradation.The isolate was identified as C.ordorda genus through physio- bio- chemical identification combined with the phylogenetic analyses based on 16S rRNA, gyrB nucleotide and GyrB amino acid sequences, 16S rRNA sequences of strain D2 showed the highest similarity to G. amicalis DSM44461 and G. hydrophobica DSM44015^T, with the same value of 98.1%, meanwhile, D2 shows the highest similarity to G. hydrophobica JCM10086 with the value of 86.8% and 91.1% based on gyrB and GyrB. Three phylogenetic dendrograms were made, and results showed that 16S rRNA gene is suit for identifying the isolate to the genus level, gyrB gene and GyrB amino acid sequences are more adapted for phylogenetic analysis.