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金鱼温和气单胞菌的分离鉴定及药敏试验 被引量:32

Isolation,identification and drug sensitive test of Aeromonas sobria from goldfish
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摘要 2006年7月从上海奉贤某一养殖基地的发病金鱼中分离出一株细菌,对它的形态、生理生化特征及16S rRNA基因序列进行了研究。结果表明,该菌为革兰氏阴性杆菌,具极生单鞭毛,发酵葡萄糖产酸不产气,氧化酶、接触酶阳性,VP阳性,生长无需NaCl,在TCBS平板上生长缓慢,对O/129弧菌抑制剂不敏感。结合16S rRNA基因序列分析结果,该菌与温和气单胞菌的亲缘关系最近,因此将该菌鉴定为温和气单胞菌(Aeromonas sobria)。同时,用20种药物对该菌进行了药物纸片扩散法抑菌试验,其中,庆大霉素、氟派酸、氯霉素、妥布霉素、卡拉霉素、壮观霉素、菌必治的抑菌效果最佳。 A pathogenic bacterium (3-1-3) was isolated from the diseased goldfish. Its morphological, physiological, biochemical characteristics and 16S rRNA sequence analysis were studied. The isolated strain is gram-negative, with polar single-flagella, glucose fermented without gas production, oxidase-positive, catalasepositive, unrequired sodium ions for growth, no pigment, non-luminescence; It grew slowly on TCBS-plate and insensitive to the vibriostratic agent O/129. The sequence analysis of 16S rRNA gene of 3-1-3 isolation and comparison with that of other related aeromonas showed that 3-1-3 was very close to Aeromonas sobria. According to morphological, physiological, biochemical characteristics and phylogenetic analysis, the isolated strain(3-1-3 ) is identified as A. sobria. The testing results by paper extension methods for drug sensitive show that, among 20 antibiotics, gentamicin, norfloxacin, chloromycotin, tobramycin, kalamycin, spectinomycin and ceftriaxone have the most inhibitive effect on the strain.
出处 《上海水产大学学报》 CSCD 北大核心 2008年第3期285-290,共6页 Journal of Shanghai Fisheries University
基金 中央级公益性科研院所基本科研业务费专项基金(中国水产科学研究院东海水产研究所2007M06)
关键词 温和气单胞菌 金鱼 16S RRNA基因 药敏试验 Aeromonas sobria goldfish 16S rRNA gene drug test
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