摘要
介绍了一种快速简便观察分析无融合生殖甜菜胚囊发育过程的方法。甜菜胚珠经过固定和脱水,用冬青油整体透明及丁香油封片,在OLYMPUS-BX51型荧光显微镜下通过荧光显微技术观察发育中的胚囊产生自发荧光,可分辨出胚囊内部结构。用FPA和4%戊二醛两种固定液固定效果不同,后者效果更佳。
The text introduces that an easy and rapid procedure has been launched for observation of embryo sac development of apomixis in the allotriploid beet. After the fixation and dehydration, the beet ovule was whole - mounted clarity by holly oil, finally sealed under coverslips with clove iol. With the OLYMPUS- BX51 microscopy procedure, the embryo sac exhibited autotluorescence and could be clearly observed.FPA and 4% glutaraldehyde as fixative produced different results but the latter seemed to be better.
出处
《中国甜菜糖业》
2008年第2期3-4,共2页
China Beet & Sugar
基金
黑龙江大学本科生开放课题实验项目:甜菜单体附加系M14品系高频传递的胚胎学机理。项目编号:056
关键词
无融合生殖
胚囊发育
整体透明
荧光显微技术
apomixis
embryo sac development
whole - motmted clarity
fluorescence microscopy
