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湖北地方小麦品种大头黄低分子量麦谷蛋白亚基基因的分子克隆

Molecular Cloning of Low Molecular Weight Glutenin Gene from Hubei Wheat Landrace ZM11217
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摘要 采用PCR方法从湖北地方小麦品种大头黄(ZM11217)中克隆得到1个低分子量麦谷蛋白亚基(LMW-GS)基因LMW-ZM11217。该基因具有LMW-GS基因的典型结构特征,编码区长度为870bp,编码288个氨基酸。推导的氨基酸序列比较结果表明,LMW-ZM11217与Glu-A3和Glu-D3位点控制的基因具有较高的相似性(最高相似性分别为82%和83%),而与Glu-B3位点控制的基因具有较低的相似性(最高相似性为74%)。 Using PCR method,the coding sequences of a low molecular weight glutenin （LMW-GS） gene, LMW-ZM11217, was isolated from Hubei wheat landrace accession ZM11217. It had a coding region of 870 bp, and encoded a mature protein of 288 amino acids, LMW-ZM11217 was a typical LMW-GS gene. Comparison of the deduced amino acid sequence showed that LMW-ZM11217 had higher similarity with genes at Glu-A3 and Glu-D3 locus （with the highest similarity 82% 83%, respectively）, whereas it had lower similarity with genes at Glu-D3 locus （with the highest similarity 74%）.
作者 房敬业 李三和 李宗瑾 刘勇 汪越胜 何光源
机构地区 华中科技大学中英HUST-RRes基因工程和基因组学联合实验室 湖北省农业科学院粮食作物研究所
出处 《湖北农业科学》 北大核心 2008年第5期489-492,共4页 Hubei Agricultural Sciences
基金 国家"973"计划项目资助课题(2002CB111301)
关键词 小麦地方品种 大头黄(ZM11217) 低分子量麦谷蛋白亚基 基因克隆 wheat landrace Datouhuang（ZM11217）：low molecular weight glutenin subunit gene cloning
分类号 S512 [农业科学—作物学] Q785 [生物学—分子生物学]
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参考文献12

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二级参考文献4

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湖北农业科学
2008年 第5期

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