铜绿假单胞菌对亚胺培南的耐药性分析及机制探讨

Imipenem-resistant Mechanism of Pseudomonas Aeruginosa

目的:研究铜绿假单胞菌对亚胺培南的耐药性及其机制。方法:取痰液中培养分离的铜绿假单胞菌55株,经K-B法检测耐药性。采用外膜蛋白图谱,对其外膜蛋白OprD2进行SDS-PAGE分析,双纸片增效法检测金属β-内酰胺酶。结果:根据耐药性分为铜绿假单胞菌亚胺培南菌耐药36株和敏感19株。铜绿假单胞菌亚胺培南耐药株不同程度OprD2减少,耐药组OprD2相对含量明显低于敏感组(P<0.01);产金属β-内酰胺酶6株,检出率10.9%。结论:OprD2的减少和产金属β-内酰胺酶是铜绿假单胞菌对亚胺培南耐药的主要原因。

OBJECTIVE： To investigate the mechanism of imipenem - resistance in clinical isolates of Pseudomonas aeruginos （PA） . METHODS： 55 strains of PA had been isolated from sputum specimen in clinic and their drug sensitivity to imipenem was tested by K- B slip diffusion method. Their amount of outer membrane protein OprD2 was analyzed by SDS - PAGE with outer membrane protein profiles. Metallo - β - lactamases （MBLs） was detected by double - disk synergy test （DDST） . RESULTS： 36 strains of imipenem - resistant and 19 strains of imipenem - sensitive PA isolated were studied. The amount of OprD2 showed a various degree of reduction in all resistant isolates, and the relative amount of OprD2 in resistant isolates were significantly lower than in sensitive isolates （P〈 0.01） . 6 （10.9%） were MBLs-producing strains. CONCLU- SIONS： The reduction of OprD2 and MBLs are the main factors accountable for Pseudomonas aeruginosas＇ s resistance to imipenem. KEY WORDS