不同显色方法对知母总皂苷含量测定的影响

Effects of Two Different Chromogenic Agents on Determination of Content of Total Saponins in Anemarrhena

目的：考察不同显色方法对紫外-可见分光光度法测定知母总皂苷含量的影响。方法：采用紫外-可见分光光度法测定知母总皂苷含量，分别用两种显色方法，显色剂方法1为香草醛-冰醋酸法。即香草醛-冰乙酸：高氯酸：冰乙酸（2：8：50）。检测波长为545nm。显色方法2为浓硫酸法，即用浓硫酸：甲醇（1：4）。检测波长为270nm。结果：采用方法1显色，知母皂苷A3在0.058～0．58mg范围内线性关系良好（r=0．9999），知母皂苷样品加样回收率分别为100．19％。RSD为2．81％；方法2显色，知母皂苷A3在0．0128～0．64mg范围内线性关系良好（r=0．9996），知母皂苷样品加样回．收率分别为98．50％，RSD为5．04％。结论：综合精密度实验、重复性实验、准确度实验等指标。方法1明显优于方法2．且该方法简便、准确、灵敏、重现性好。

Objective： To investigate the effects of two different chromogenic agents on determination of the content of total sap- onins in Anemarrhena by UV spectrophotometry. Methods：Total Anemarrhenasaponin were determinated by two different chromo- genie agents, namely method one, vaniUin - glacial acetic acid perehlorle acid glacial acetic acid （2： 8：50）at 545 nm;method two, sulfuric acid- methanol（ 1：4 ） detected at 270 nm. Results：Using method one, the linear range of Anemarrhenasaponin A3 was 0. 058 ~0. 58 mg（r =0. 9999）. The recovery rate was 100. 19% ,RSD2. 81% ; using method two,the linear range of Anemarrhe- nasaponin A3 was 0. 0128 - 0.64 mg（ r = 0. 9996 ）. The recovery rate was 98.50% , RSD4. 96%. Conclusion ： From these index, the first method was better than the second method. This method was simple ,accurate and the reproducibility was good.