酿酒酵母产孢培养基的筛选及单倍体的分离

The Screening of Sporogenous Medium and Haploid Separation of Saccharomyces Cerevisiae

为获得酿酒酵母1450遗传育种单倍体亲本,采用不同的培养基对酿酒酵母1450进行了生孢培养实验,通过优化得出,葡萄酒酵母1450的最适宜的产孢条件是,Mcclary培养基于25～28℃条件下培养6 d,在此条件下,酿酒酵母1450的产孢率为70%左右。子囊孢子分离得到单倍体菌株,通过测定这些单倍体菌株的发酵力和发酵性能,筛选出单倍体S-3为一株优良的酿酒酵母1450遗传育种单倍体亲本。

To obtain a haploid genetics hybrid breeding of 1450 （S.Cerevisiae）, Spore-producing cultivation experiments of 1450 were done by using different culture media and under different conditions. The results showed that the optimum sporulation medium and cultivation time were McClary medium and 6 days, the cultivation temperature must be controlled at 25～28℃. Under the above conditions, the sporulation rate could reach to about 70 %. A yeast strain S-3 as a genetics hybrid breeding was isolated by comparasion of the fermentation capability and performance of haploid.