摘要
[目的]观察桃核承气汤对蓄血证大鼠血管基质金属蛋白酶-2(Matrix Metalloproteinase-2,MMP-2)及金属蛋白酶-2组织抑制剂(Tissue Inhibitor of Metalloproteinase-2,TI MP-2)基因表达的影响,探讨此方的作用机制。[方法]半定量RT-PCR检测各组大鼠颈总动脉匀浆中MMP-2、TI MP-2基因的表达,进行分析。[结果]与模型组相比,药物组减少了MMP-2基因的表达,增加了TI MP-2的表达(P<0.01)。[结论]桃核承气汤能改变蓄血证大鼠动脉壁MMP-2、TI MP-2基因的表达,这可能是其主要作用机制之一。
[Objective]To investigate the effects of Taohechengqitang(THCQT) on the gene expression of Matrix Metalloproteinase- 2(MMP-2) and Tissue Inhibitor of Metalloproteinase-2(TIMP-2)in artery vessel of Xuxuezheng rats. [Methods] Half quantitative reverse transcription polymerase chain reaction(RT-PCR) was used to detect MMP-2 and TIMP-2 gene expression in artery vessel. They were analyzed. [Results]While compared with model group, MMP-2 gene expression levels in the THCQT group were lower, and TIMP-2 was higher (P〈0.01). [Conclusion]THCQT can change the gene expression of MMP-2 and TIMP-2 in artery vessels of Xuxuezheng rats, which may account for the effects.
出处
《浙江中医药大学学报》
CAS
2008年第1期38-40,共3页
Journal of Zhejiang Chinese Medical University
基金
浙江省教育厅科研基金资助项目(No:20050828)~~