姜黄素对神经病理性痛大鼠脊髓背角c-fos表达的影响

Effects of Curcumin on Behavior and c-fos Expression in Spinal Cord in Chronic Constrictive Injury Rats

[目的]观察姜黄素对神经病理性痛大鼠痛行为及脊髓背角原癌基因c-fos表达的影响。[方法]采用大鼠坐骨神经慢性压迫性损伤(CCI)模型。雄性SD大鼠108只,随机分为实验对照组、假手术组、溶剂对照组、姜黄素组。采用vonFrey纤维丝和热痛刺激仪测定大鼠热痛缩爪潜伏期(TWL)和机械性缩爪潜伏期(MWT),在术后3、7、14天处死(n=6),用免疫组织化学方法测定c-fos蛋白在脊髓背角神经元的动态变化。[结果]CCI大鼠术侧脊髓背角浅层内Fos阳性神经元明显增多,姜黄素可减轻神经病理痛大鼠的痛行为,也能够明显减少脊髓背角神经元中Fos表达。[结论]姜黄素能减轻神经病理性痛,其机制可能与降低Fos蛋白的合成有关。

[Objective]To investigate the effects of curcumin on the behavior of chronic constrictive injury（CCI） rats and the c-fos expression in spinal cord dorsal horn. [Methods]108 male SD rats were randomly divided into 4 groups; Control group（treated with CCI） ; Sham operation group; Solvent contrast group; Curcumin treated group. Thermal withdrawal latency（TWL） and mechanical withdrawal threshold（MWT） of rats were determined by Hargreave＇s thermal radiation apparatus and yon Frey＇s method of microfilament, respectively. The time course of expression of c-los were assessed by immunohistochemical analysis. [Results] Curcumin could significantly attenuate the activation of c-fos induced by CCI and simultaneously ameliorate thermal and mechanical hyperalgesia induced by CCI. [Conclusion]This study shows that curcumin could attenuate the activation of c-fos to ameliorate the CCI：induced neuropathic pain.