CS-HPLC-ESI-MS^n法分析复方丹参滴丸的代谢指纹图谱(英文)

Metabolic fingerprint and profile analysis of compound Danshen dripping pills by CS-HPLC-ESI-MS^n

目的:建立舌下含服复方丹参滴丸后人血浆中活性成分的代谢特征指纹图谱。方法:在不同时间点采集人血浆样品,通过微血管张力实验测试血管扩张效果,采用柱切换系统对样品进行富集纯化。结果:1.5h采集的血浆扩张血管能力最强,在色谱图中出现5个新的色谱峰。结论:色谱图中5个特征色谱峰与复方丹参滴丸的药效物质基础关系密切。

Aim： To establish the metabolic fingerprint and profile of bioactive components in human plasma after sublingual administration of compound Danshen dripping pills. Methods： Human plasma samples were collected at different time intervals. Powerlab tension system was used to measure their ability of expanding blood vessel and column switching system was employed to purify and enrich the components of interest. Results： The plasma sample of 1.5 h had the strongest ability for expanding blood vessel, and had five new peaks in the chromatogram. Conclusion： After being identified, the intensity ratio of these 5 peaks was calculated. The diagram called metabolic profile was established to link bioactive compounds with pharmacodynamic action.