绿色荧光蛋白基因转染内皮前体细胞与肿瘤新生血管的关系

Relationship between GFP-gene-transfected endothelial progenitor cells and tumor angiogenesis

目的:了解骨髓来源的内皮前体细胞(endothelial progenitor cells,EPCs)与肿瘤新生血管的关系。方法:通过分离、培养骨髓来源的EPCs,真核表达载体pcDNA3.1/GFP质粒以阳离子脂质体转染法将其导入EPCs,并检测其表达;建立小鼠Lewis肿瘤移植模型,回输基因修饰EPCs,免疫组织化学法观察肿瘤新生血管局部GFP表达情况并计数。结果:pcDNA3.1/GFP真核表达载体转染EPCs后,EPCs表达GFP片断。部分GFP标记的EPCs在肿瘤新生血管定位,计数结果提示约14.0%肿瘤新生血管内皮细胞来源于EPCs。结论:骨髓来源的EPCs参与肿瘤新生血管形成。

Aim： To reveal the relationship between endothelial progenitor cells （EPCs） and angiogenesis in tumor. Methods： EPCs derived from bone marrow were isolated and identified; the pcDNA3. 1/GFP plasmid eukaryotic expression vector in cationic liposomes transfected EPCs, then reinfused into the Lewis tumor-bearing mice; the GFP expression was observed in the location of tumor by immunohistochemistry and the number of GFP positive endothelium in the angiogenesis area was counted. Results： After being transfected with pcDNA3.1/GFP plasmid, EPCs expressed GFP fragment. Part of EPCs expressed GFP fragment was found to locate in the area of the angiogenesis during the tumor growth process, counting results showed that about 14% of endothelium in tumor new blood vessel expressed GFP. Conclusion： It proves that bone marrow derived EPCs contribute to angiogenesis of tumor.