超声心动图观察杠柳毒苷对慢性心衰大鼠左室结构和功能的影响

Effect of glucoperiplocymarin on left ventricular structure and function in chronic heart failure rats by color doppler-echocardiography

[目的]应用彩色超声心动图评价杠柳毒苷对慢性心衰大鼠左室结构和功能的影响。[方法]采用结扎大鼠冠状动脉前降支法造成心肌梗死后慢性充血性心力衰竭(CHF)模型,随机分为假手术组、模型组、地高辛组、杠柳毒苷8、4、2 mg/kg剂量组。给药4周后,分别测定左室舒张末期内径(LVDd),左室收缩末期内径(LVDs)、左室舒张末期室间隔厚度(LVSd)、左室收缩末期室间隔厚度(LVSs)、左室舒张末期后壁厚度(LVPWd)、左室收缩末期后壁厚度(LVPWs);左室射血分数(EF)、左室短轴缩短率(FS)、左室舒张末期容积(LVEDV)、左室收缩末期容积(LVESV)、每搏心排血量(SV)。[结果]各给药组LVDd、LVDs均有不同程度的缩小,其中杠柳毒苷8 mg/kg剂量组、地高辛组与模型组比较有显著性差异(P<0.05,P<0.01);各给药组均可以升高EF、FS,降低LVEDV、LVESV,与模型组相比,地高辛组均有显著性差异(P<0.05,P<0.01),其中杠柳毒苷8、4 mg/kg剂量组EF和LVESV,杠柳毒苷8 mg/kg剂量组FS与模型组比较差异具有显著性(P<0.05,P<0.01)。[结论]杠柳毒苷治疗可以改善慢性心衰大鼠左室结构和功能,8 mg/kg剂量组有优于其他组的趋势。

[ObJective] To observe the effect of Glucoperiplocymarin on left ventricular structure and function in chronic heart failure（CHF） rats by Color Doppler-echocardiography. [Methods] The model of CHF was induced by ligating the anterior descending branch of the left coronary artery. The rats were at random divided into six groups： sham operation group, model group, digoxin group, three treatment groups treated with 8 mg/kg, 4 mg/kg and 2 mg/kg dosage of Glucoperiplocymarin. After administration for 4 weeks, The effect of Glucoperiplocymarin treatment on LVDd, LVDs, LVSd, LVSs, LVPWd, LVPWs, EF, FS, LVEDV, LVESV, SV was observed. [Results] All the treated groups could decrease LVDd and LVDs in different degree, and there was significant difference between the Glucoperiplocymarin 8 mg/kg dosage treated group,digoxin group and model group （P〈0.05, P〈0.01 ） ;All the treated groups could increase EF,FS and decrease LVEDV and LVESV. Compared with model group, EF and LVESV of the Glu- coperiplocymarin 8 mg/kg,4 mg/kg dosage treated group and FS of the Glucoperiplocymarin 8mg/kg dosage treated group had significant difference（P〈0.05, P〈0.01）. [Conclusion] Glucoperiplocymarin could improve the left ventricular structure and function in chronic heart failure rats. There was a trend that the effects of 8mg/kg dose group of the Glucoperiplocymarin were better than others.