摘要
以氨基修饰的磁性SiO2纳米粒子为载体,通过交联剂戊二醛固定漆酶,对固定化条件进行了优化,比较了固定化酶与游离酶的酶学性质.结果表明,漆酶固定化的最佳条件为戊二醛浓度8%(ω),固定化时间6h,缓冲液pH值7.0,初始酶液浓度0.15g/L.固定化的漆酶的最适pH为4.0,最适温度为20℃.在60℃条件下保温4h,固定化漆酶仍能保持酶活力60.9%,在连续10次操作后,酶活力仍能保持55%以上,其热稳定性和操作稳定性均比游离酶高.
Laccase was immobilized on the silanized magnetic silica nanoparticles by using glutaradehyde as the cross-linker. Immobilization of laccase was investigated and its immobilizing conditions were optimized. The results were achieved with the concentration of 8% glutaradehyde, immobilizing time of 6 h, the buffer of pH 7.0 and initial laccase concentration of 0.15 g/L. The optimum pH and temperature of immobilized laccase were 4.0 and 20 ℃, respectively. After the immobilized enzyme was kept at 60 ℃ for 4 h, it remained 60.9% of its initial activity. The immobilized laccase could retain above 55% of activity after 10 consecutive operations. The experimental results showed that the thermal stability and operational stability of immobilized laccase were improved in comparison with free laccase.
出处
《过程工程学报》
EI
CAS
CSCD
北大核心
2008年第3期583-588,共6页
The Chinese Journal of Process Engineering
基金
国家高技术研究发展计划(863)基金资助项目(编号:20060102Z2049)