线艺建兰的组织培养和快速繁殖(简报)

Tissue Cultrue and Rapid Propagation of Cymbidium ensifolium with Verge Line Pattern Leaves

以线艺建兰茎尖、腋芽为外植体,接种在1/2MS+6-BA3.0mg/L+NAA0.3mg/L培养基上形成原球茎,原球茎在1/2MS+6-BA0.1mg/L+NAA1.0mg/L培养基上可大量增殖,并伸长生长形成丛生型根状茎;根状茎在MS+6-BA2.0mg/L+PP3331.0mg/L+NAA0.5mg/L培养基上可分化成小苗,分化率达46.3%;小苗在MS+IBA1.0mg/L+GA0.5mg/L+香蕉泥100g/L的培养基上生根壮苗,生根率达100%。