Smad7蛋白分子及IFN-γ在人膀胱移行上皮细胞癌中表达的临床意义

The expression of Smad7 and IFN-γ in human transitional cell carcinoma of bladder and its clinical significance

目的:探讨Smad7和IFN-γ在人膀胱移行上皮细胞癌(TCC)中表达的临床意义和两指标之间的关系。方法:采用免疫组织化学SP法对52例人膀胱TCC和10例正常膀胱组织中的Smad7和IFN-γ的表达进行检测,用光镜对表达结果进行定性分析,采用图像分析进行定量分析。结果:定性分析表明膀胱癌组织中表达的Smad7和IFN-γ两指标之间有联系(P<0.01)。定量分析表明Smad7和IFN-γ在膀胱癌组中的阳性表达显著高于正常膀胱组(P<0.01);Smad7和IFN-γ的表达与膀胱癌的细胞病理分级,临床分期有关(P<0.01)。结论:TCC中Smad7和IFN-γ随细胞病理分级,临床分期增高阳性表达程度增强,提示Smad7在人膀胱TCC的发生发展中起重要作用,而IFN-γ作为一种广泛的非特异性的免疫调节因子与Smad7存在潜在的联系机制。

Objective：To investigate the clinical significance of expression of Smad7 and IFN-γ in human transitional cell carcinoma of bladder（TCC） and the correlation between Smad7 and IFN-γ. Methods： The expression of Smad7 and IFN-γ in TCC tissues of 52 cases and normal bladder tissues of 10 cases were studied by immunohistochemical staining and qualitative analysis of microscope and quantitative analysis of image. Results： Qualitative analysis demonstrated that the expression of Smad7 contacts with the expression of IFN-γ in TCC tissues（P〈0.01）. Quantitative analysis demonstrated that the expression of Smad7 and IFN-γ in TCC is higher than those in normal bladder tissues of controls and the expression of Smad7 and IFN-γ have a positive correlation with both cell grade and T stage（P〈0.01 ）. Conclusion：With the rise of cell grade and T-stage the positive expression degree of Smad7 and IFN-γ in TCC tissues goes up. The upregulation of Smad7 suggests that it plays an important role in the for mation and development of TCC and IFN-γ as a general immunological modulator factor potentially contacts with Smad7.