宫内给药对晚孕大鼠细菌性宫内感染的治疗研究

The Effect of Antibiotics through Amniocentesis against Intrauterine Infection in Late Pregnant rats

目的:观察头孢曲松钠宫内给药对晚孕大鼠细菌性宫内感染的治疗作用。方法:孕15天SD雌鼠36只,随机等分4组,各鼠一侧宫角接种大肠埃希菌。孕16天,羊膜腔内给药组和羊膜腔内对照组接种侧各胎囊内注药和等体积0.9%氯化钠液,尾静脉给药组和静脉对照组尾静脉注药和等体积0.9%氯化钠液。孕17天起每组每天随机处死3只,计算死胎率与接种侧顶端3个胎囊羊水大肠埃希菌阴性率和胎盘轻度炎症率。结果:羊膜腔内给药组较羊膜腔内对照组总体和给药后3天死胎率低,大肠埃希菌阴性率高(P<0.05)。羊膜腔内给药组与尾静脉给药组相比,总体和给药后1天大肠埃希菌阴性率、胎盘轻度炎症率均高(P<0.05)。结论:宫内头孢曲松钠单次给药治疗晚孕大鼠大肠杆菌宫内感染能更快抑菌和减轻胎盘炎症,减少死胎。

Objective：To explore the effect of ceftriaxone sodium in treatment of intrauterine infection in late pregnant rats. Methods：36 SD pregnant rats of 15 days of pregnancy were randomly divided into four groups. E coli suspension was inoculated into one uterine horn of each rat. On 16th day of pregnancy. Ceftrlaxone were injected into amniotic cavity of group one （IUA） through amniocentasis, while same volume of saline were administrated the same way in group two as control （IUC）. Ceftriaxone were injected though tail veins in group three （IVA）, and same amount of saline were administrated the same way in group four as control （ IVC）. From the 17th day, 3 rats of each group were killed randomly everyday. Still birth rate will be calculated. Amniotic fluid and placenta infection were examed. Results：The still birth rates in total and on the 3rd day after administration were lower in IUA than IUC, with higher E coli negative rates（ P〈 0.05）. The E coli negative rates and mild chorioamnionitis rates of IUA in total and on the 1st day after administration were higher than those of IVA（ P 〈 0.05）. Conclusions： Single dose of Ceftrlaxone sodium administered in late pregnant SD rats can inhibit E coli growth and relieve chorioamnionitis mere quickly, and thus decrease still birth rate.