α-双炔失碳酯对激素依赖人前列腺癌细胞LNCaP生长的影响

Effects of α-Anordrin on the Growth of Androgen-Dependent Human Prostate Cancer Cell Line LNCaP

目的探讨双炔失碳酯α单体(α-anordrin,α-ANO)对激素依赖人前列腺癌细胞LNCaP生长的影响。方法采用氧化铝柱层析分离纯化双炔失碳酯(ANO)的α和β差向异构体,SRB细胞染色法检测α-ANO对LNCaP细胞生长的影响及对抗雄激素拟似剂R 1881促细胞生长的作用;观察α-ANO作用下细胞形态学变化;ELISA法检测α-ANO对LNCaP细胞分泌前列腺特异性抗原(PSA)的影响。结果常规培养液中α-ANO(8,12,16,24,32μmol/L)作用LNCaP细胞后,细胞存活率分别为85.8%,51.5%,40.8%,29.2%,1.0%;在去除激素培养液中,α-ANO抑制细胞生长作用更强,能对抗R1881的促细胞生长作用,经α-ANO处理的LNCaP细胞分泌PSA能力降低。结论α-ANO抑制激素依赖人前列腺癌细胞LNCaP的增殖,具有抗雄激素促细胞生长的作用。

Objective To investigate the effects of α-anordrin（α-ANO）on the growth of androgendependent human prostate cancer cell line LNCaP. Methods The monomers of α-ANO and β-ANO were purified through alumina column chromatographic separation. SRB staining method was used to detect the effects of α-ANO on the growth of LNCaP cells and on antagonizing R1881（a kind of androgen stimulant） in inducing LNCaP cells proliferation. Cell morphology was examined under microscope. ELISA was used to examine the effect of α-ANO on PSA secretion by LNCaP cells. Results In normal medium, after LNCaP cells were treated with α-ANO at 8,12,16,24 and 32 μmol/L, cell survival rates were 85.8%, 51.5%, 40.8%, 29.2%, 1.0% respectively. In the androgen-depleted medium, α-ANO could antagonize LNCaP cell proliferation induced by R1881. The PSA secretion of LNCaP cells was also decreased. Conclusion α-ANO inhibits the proliferation of androgen-dependent human prostate cancer cell line LNCaP. In the presence of androgen, α-ANO antagonizes the LNCaP cell proliferation induced by androgen.