湖北地区汉滩病毒与普马拉病毒及多布拉伐病毒的血清学交叉反应研究

Humoral cross-reactivity of hantaan viruses to the nucleocapsid protein of Puumala viruses and Dobrava viruses in Hubei province

目的研究湖北地区汉滩病毒(hantaan virus,HTNV)与普马拉病毒(Puumala virus,PUUV)、多布拉伐病毒(Dobrava virus,DOBV)血清学交叉反应。方法以酵母菌表达的PUUV、DOBV、HTNV重组核衣壳蛋白(recombinant nucleocapsid protein,rNP)为包被抗原,ELISA法定性、定量分析湖北地区HTN型急性/恢复期血清中IgA、IgG、IgM抗体,比较疾病早晚期血清学交叉反应变化。结果DOB-rNP与HTN型血清中IgA、IgG、IgM抗体交叉反应性较高,PUU-rNp对HTN型血清交叉反应很低;IgA、IgG抗体交叉反应在恢复期血清中均增高,且IgA抗体交叉反应更多更强。结论湖北地区HTN型血清与DOBV存在广泛交叉反应,与PUUV交叉反应少。各交叉反应在恢复期增高对汉滩病毒血清学研究具重要意义。

Objective To study the cross-reactivity of hantaan vires to recombinant nucleocapsid proteins of Puumala virus and Dobrava vires in Hubei province. Methods Six yeast-expressed recombinant nucleocapsid proteins of hantavimses, which were derived from PUUV Kazan strain, PUUV Vranica strain, PUUV Sotkamo strain, DOBV Slovakia strain, DOBV Slovenia strain, and HTNV Fojnica strain, were extracted and purified as coated antigens for ELISA assay. The indirect ELISA assay was performed to detect the cross-reacting IgA, IgG and IgM in 34 pairs of acute/convalescent serum samples collected from patients with hemorrhagic fever with renal syndromes （HFRS） in Hubei province. All the samples were distinguished as HTNV by RT-PCR genotyping. Qualitative and quantitative results of ELISA assay were analyzed to compare the change of cmss-reactivities between the acute and convalescent phase of disease. Results Sera of the patients infected with Hantaan viruses showed a strong cross-reactivity to DOB-derived rNP while a weak cross-reactivity to the PUU-derived proteins. There were marked increases of IgA and IgG cross-reactivities between the hetemlogous hantavims recombinant nucleocapsid proteins and HTN-positive sera in convalescent phrase. Moreover, the increase of IgA cross-reactivity was much stronger and more significant. Conclusion HTN-positive sera collected from Hubei province are cross-reacted strongly to DOB-derived recombinant nucleocapsid proteins in IgA, IgG, and IgM responses, while the cross-reactivity to the PUU-derived proteins is weak. The increase of IgA and IgG cross-reactivity to hetemlogous hantavimses in convalescent sera has significant implications for serological study of hantaan viruses.