periostin在人瘢痕成纤维细胞中的表达及氢化可的松对其表达的影响

Expression of periostin and the effect of hydrocortisone on it in human fibroblasts of scar

目的:通过检测periostin在人过度增生性瘢痕成纤维细胞中的表达及其对氢化可的松作用的反应,探讨periostin在瘢痕形成中的作用。方法:采用组织块培养法体外培养原代成纤维细胞,以RT-PCR及免疫细胞化学技术分别检测24例人瘢痕和正常皮肤成纤维细胞中periostin的mRNA和蛋白的表达,氢化可的松(0.1 g/L)的作用时间为96 h。结果:periostin的mRNA在瘢痕疙瘩成纤维细胞(1.645±0.549)和增生性瘢痕成纤维细胞(1.084±0.396)中的表达均高于正常皮肤成纤维细胞(0.274±0.215;P<0.05),两者分别增加83%和75%。氢化可的松作用后,瘢痕疙瘩成纤维细胞中和增生性瘢痕成纤维细胞中periostin的mRNA表达量分别减少32%和47%,两者与氢化可的松作用前相比差异均有统计学意义。Periostin蛋白主要分布在成纤维细胞核周围的胞浆中,它在瘢痕疙瘩和增生性瘢痕的成纤维细胞中的表达(分别为91.815±0.961和70.166±2.250)高于在正常皮肤成纤维细胞中的表达(41.011±1.576,P<0.01),两者分别增加55%和42%。结论:periostin在过度增生性瘢痕成纤维细胞中的表达增高,氢化可的松可能抑制其基因的表达;periostin可能影响过度增生性瘢痕成纤维细胞的功能,它可能是一种瘢痕相关基因。

Objective：To probe into the role of periostin in the formation of scars and investigate its reaction to hydrocortisone. Methods： Primary fibroblasts were cultured in vitro, then RT-PCR and immu- nocytochemical technique were used to examine the expressions of mRNA and preotein of periostin respec- tively in 24 samples of keloid fibroblasts （ KFb）, hypertrophic scar fibroblasts （HFb） and normal skin fi- broblasts （SFb）. Results： The mRNA levels of periostin in KFb （ 1. 645 ± 0. 549 ） and HFb （ 1. 084 ± 0. 396） were both higher than that in SFb （0. 274 ±0. 215, P 〈0.05）. After hydrocortisone was added, the mRNA levels ofperiostin in KFb and HFb were both decreased by 32% and 47% respectively （P 〈 0. 05 ） ; The protein of periostin distributed mainly in the endochylema around the nucleus of fibroblasts. The protein levels ofperiostin in KFb （91. 815 ±0. 961 ） and HFb （70. 166 ±2. 250） were both higher than that in SFb （41. 011 ± 1. 576, P 〈0. 01 ）. Conclusion： The expressions ofperiostin were increased in the fibroblasts of hyperplasic scars, which could be inhibited by hydrocortisone, periostin may affect the function of fibroblasts of hyperplasic scars, periostin may be a cicatrix specific gene.