NGAL表达与乳腺癌进展、转移及预后的关系

Relationship between NGAL expression and progression,metastasis and prognosis of breast cancer

目的探讨中性粒细胞相关载脂蛋白(NGAL)在乳腺癌组织中的表达及与乳腺癌进展、转移及预后的关系。方法应用免疫组化方法检测NGAL在127例乳腺癌,20例乳腺良性肿瘤组织中的表达,分析其与乳腺癌临床病理特征及预后的关系。结果NGAL在乳腺癌组织中的阳性表达率为40.2%,明显高于在乳腺良性肿瘤中的表达(χ2=6.816,P=0.009),NGAL表达与乳腺癌组织学分级(χ2=6.269,P=0.012)和病理类型(χ2=14.213,P=0.001)有关,与TNM分期(r=0.192,P=0.031)、淋巴结转移(r=0.257,P=0.004)和HER-2(r=0.287,P=0.001)呈正相关,与ER(r=-0.197,P=0.026)呈负相关。NGAL表达阳性组和阴性组的5年特异生存率分别为38.4%和63.4%,差异有显著性(P=0.016)。结论NGAL阳性表达提示乳腺癌预后不良。NGAL可作为评价乳腺癌预后新的生物学指标,为乳腺癌综合治疗提供依据。

Objective To investigate the expression of neutrophil gelatinase-associated lipocalin （NGAL） and to evaluate its relation with progression, metastasis and prognosis of breast cancer. Methods The expression of NGAL was detected in 127 breast cancer tissues and 20 benign tumor tissues by immunohistochemistry and its correlation with clinicopathological parameters and prognosis was analyzed. Results The positive rate of NGAL expression in breast cancer was 40. 2%, which was significantly higher than that in benign tumor tissues of breast （x^2= 6. 816, P= 0. 009）. NGAL expression was related to histologic grade （x^2= 6. 269, P = 0.012） and pathological type （x^2= 14. 213, P = 0. 001）. The expression of NGAL was observed to correlate positively with TNM stage （r= 0. 192, P= 0. 031）,lymph node status （r= 0.257, P = 0.004） and HER-2 status （r= 0. 287, P = 0. 001 ）, and correlate negatively with ER status （r = - 0. 197, P = 0. 026 ）. In addition, Kaplan-Meier curves of disease-specific survival analysis showed a significant difference between NGAL positive groups and negative group （P=0. 016）. Conclusion NGAL might be a poor prognostic factor for human breast cancer, and also might be a novel molecular marker to predicate the prognosis and to guide the comprehensive treatment of breast cancer.