肺癌组织蛋白激酶C活性和亚型的测定及其临床意义

Detection of protein kinase C and its isoform expression in lung cancer tissues and its clinical significance

目的 研究肺癌组织蛋白激酶C活性、亚型及其临床意义。方法 从23例肺癌患者4种类型的新鲜组织取材，测定其蛋白激酶C活性及亚型，并与正常肺组织作比较。结果 肺癌组织蛋白激酶C活性明显高于正常肺组织（t=2．46，P〈0．05），而且以细胞膜的升高更为明显（t=4．63，P〈0．01），表现出典型的转膜现象；4种类型肺癌组织的蛋白激酶C活性未见统计学差异（P〉0．05）。肺癌各年龄组、吸烟组与非吸烟组的蛋白激酶C活性无统计学差异（P〉0．05）。按pTNM分期比较，自Ⅰ期～Ⅲb期，细胞质和细胞膜的蛋白激酶C活性逐渐升高，但各期间差异无显著性（P〉0．05）。肺癌组织蛋白激酶C活性随分化程度增高而降低，以未分化癌为最高。另外，蛋白激酶C—a在肺癌组织胞浆的表达较正常肺组织胞浆明显增高（P〈0．05）。结论 蛋白激酶C活性显著增高可能在肺癌发生中有重要作用，可能是一种判断肺癌进展和分化程度的重要标志物。

Objective To assess the clinical significance of protein kinase C（PKC） in lung cancer tissues. Methods Fresh lung cancer tissues of four pathological types were collected in 23 cases, and the PKC activity and isoform were measured and compared with those of normal lung tissues. Results Both cytoplasm and membrane fractions of lung cancer tissues exhibited higher PKC activity than those of normal tissues （t = 2.46, P （0.05）, and more obvious in membrane fractions （t= 4.63, P〈 0. 01）, which showed translocation from cytoplasm to membrane ; PKC activity had no significant difference among the four pathological types （P〉0. 05）. There was no obvious difference in different age groups,between smokers and nonsmokers; PKC activity in cytoplasm and membrane fractions increased gradually from pTNM Ⅰ to Ⅲ b phase, but no obvious difference was showed between different phase. In addition, PKC activity decreased following the increase of differentiation level, the highest was in undifferentiated cancer. Protein kinase C-a is more higher in plasm of lung cancer tissues than that of normal lung tissues（P〈0.05）. Conclusion PKC plays a significant role in lung carcinogenesis,and it may be one of the most important cancer markers to judge the progression and differentiation degree of lung cancer.