肿瘤坏死因子受体2基因多态性与汉族人群冠心病的相关性研究

The association between tumor necrosis factor receptor 2 gene polymorphism and development of coronary artery disease

目的探讨肿瘤坏死因子受体2（tumor necrosis factor receptor2，TNFR2）第6外显子196位点基因多态性与冠心病的相关性。方法采用病例对照研究的方法，用ShineRoar探针技术检测142例冠心病患者、215例冠状动脉粥样硬化患者和631名健康体检者的TNFR2第6外显子196位点的基因型。结果TNFR2第6外显子196位点GG型基因携带者发生冠心病的风险（OR=2．556，95％CI：1．051～6．218，Χ^2=4．57，P=0．033）及冠状动脉粥样硬化的风险（OR=2．547，95％CI：1．162～5．579，Χ^2=5．81，P=0．016）明显高于TT型基因携带者；但两分类Logistic回归分析结果显示，在控制性别和年龄不变的情况下，TNFR2第6外显子GG型基因与冠心病（OR=0．614，95％CI：0．166～2．279，Χ^2=0．53，P=0．466）及冠状动脉粥样硬化（OR=0．644，95％CI：0．200～2．069，Χ^2=0．55，P=0．459）的关联无统计学意义。结论汉族人群TNFR2第6外显子196位点基因多态性不是汉族人发生冠心病及冠状动脉粥样硬化的独立风险因素。

Objective To study the association of biallelic polymorphism at position 196 in exon 6 of tumor necrosis factor receptor 2 （TNFR2） gene and coronary artery disease （ CAD）. Methods A retrospective case control study was designed in this investigation. Patients with angiographicaliy proven CAD （n = 142） and coronary atherosclerosis （n = 215） were compared to healthy volunteers （n =631）. Genotyping was performed by ShineRoar probes analysis. Results People with GG genotype at position 196 in exon 6 of TNFR2 gene have more risks of development of CAD（ OR = 2. 556, 95% CI： 1. 051 - 6. 218, Χ^2 = 4.57, P = 0. 033 ） and CA （OR = 2. 547,95 % CI：1. 162 - 5. 579, Χ^2 = 5.81,P = 0. 016） than those with TT genotype. Further analysis after adjustment for sex and age demonstrated that GG genotype was not associated with CAD （ OR = 0. 614,95% CI： 0. 166 - 2. 279, Χ^2 = 0. 53, P = 0. 466 ） and coronary atherosclerosis （OR =0. 644,95% CI：0. 200 -2. 069, Χ^2 =0. 55,P =0. 459）. Conclusion Tumor necrosis factor receptor 2 gene polymorphism at position 196 in exon 6 was not an independent risk factor of development of CAD and coronary atherosclerosis among Han Chinese.